The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 20 µg/ml.
Use a concentration of 5 µg/ml.
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 147 kDa (predicted molecular weight: 147 kDa).
RelevanceCADPS2 (Ca2+-dependent activator protein for secretion) belongs to the CAPS/Cadps family and consists of two members, CAPS1 and CAPS2. Both members play an important role in secretory granule exocytosis. CADPS2 is involved in the release of two neurotrophins, brain-derived neurotrophic factor and neurotrophin-3, from parallel fibers of cerebellar granule cells. CAPS proteins are expressed predominantly in the brain. CADPS2 has been found in various brain regions including the olfactory bulb, cerebrum, hippocampal formation, thalamus, mesencephalic tegmentum, cerebellum, medulla and spinal cord. CADPS2 colocalizes with proteins related to exocytosis (VAMP and SNAP-25) and endocytosis (Dynamin I) in the cell soma and processes of the mesencephalic tegmentum and cerebellum.
Cellular localizationCytoplasmic. Membrane-associated to vesicles.