Overview

  • Product name
  • Description
    Rabbit polyclonal to CAIN
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1000 - 1100 of Human CAIN.

    (Peptide available as ab88931.)

  • Positive control
    • This antibody gave a positive signal in Mouse Skeletal Muscle Tissue Lysate, and in the following whole cell lysates: SHSY-5Y; SK N SH; TE 671.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 246 kDa (predicted molecular weight: 246 kDa).

Target

  • Function
    May be required for replication-independent chromatin assembly. May serve as a negative regulator of T-cell receptor (TCR) signaling via inhibition of calcineurin. Inhibition of activated calcineurin is dependent on both PKC and calcium signals.
  • Tissue specificity
    Widely expressed in different tissues.
  • Sequence similarities
    Contains 6 TPR repeats.
  • Post-translational
    modifications
    Activated through PKC-mediated hyperphosphorylation.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • A330070M20Rik antibody
    • Cabin 1 antibody
    • CABIN_HUMAN antibody
    • Cabin1 antibody
    • CAIN antibody
    • Calcineurin binding protein cabin 1 antibody
    • Calcineurin inhibitor antibody
    • Calcineurin-binding protein cabin-1 antibody
    • KIAA0330 antibody
    • PPP3IN antibody
    see all

Images

  • All lanes : Anti-CAIN antibody (ab76600) at 1 µg/ml

    Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 2 : SK N SH (Human neuroblastoma) Whole Cell Lysate
    Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 4 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 246 kDa
    Observed band size : 246 kDa
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References

This product has been referenced in:
  • Müller S  et al. Phosphorylation and DNA binding of HJURP determine its centromeric recruitment and function in CenH3(CENP-A) loading. Cell Rep 8:190-203 (2014). WB . Read more (PubMed: 25001279) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting us. We are sorry; ab57137 is no longer included in our product range. We apologize for the inconvenience caused. Could we recommend few other products from our catalogue? We have ab3349 and ab76600 antibodies available ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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