Overview

  • Product name
    Cal-520, AM
  • Tested applications
    Suitable for: Functional Studiesmore details
  • General notes

    ab171868 provides a robust homogeneous fluorescence-based assay tool for detecting intracellular calcium mobilization. Cal-520 AM is a new fluorogenic calcium-sensitive dye with a significantly improved signal to noise ratio and intracellular retention compared to the existing green calcium indicators (such as Fluo-3 AM and Fluo-4 AM). Cells expressing a GPCR or calcium channel of interest that signals through calcium can be preloaded with Cal-520 AM which can cross cell membrane. Once inside the cell, the lipophilic blocking groups of Cal 520 AM are cleaved by esterases, resulting in a negatively charged fluorescent dye that stays inside cells. Its fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with agonists, the receptor signals the release of intracellular calcium, which significantly increase the fluorescence of Cal-520. The characteristics of its long wavelength, high sensitivity, and >100 times fluorescence enhancement, make Cal-520 AM an ideal indicator for the measurement of cellular calcium. The high S/N ratio and better intracellular retention make the Cal-520 calcium assay a robust tool for evaluating GPCR and calcium channel targets as well as for screening their agonists and antagonists.

    Molecular Weight (MW) 1102.95 Da.

Properties

Applications

Our Abpromise guarantee covers the use of ab171868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • CHO-M1 cells were seeded overnight at 40,000 cells per 100 µL per well in a 96-well black wall/clear bottom plate. 100 µl of 4 µM Cal 520 AM in HHBS were added into the wells, and the cells were incubated at 37 °C for 2 hour.

Protocols

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

Very good signal

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Cal-520 used for calcium imaging on primary astrocyte culture.
Used following the protocol provided and imaged under a Leica Sp6.

Figure included is an example of image when astrocytes are stimulated with ATP (50uM).
Username

Ms. Maria Velasco

Verified customer

Submitted Jun 05 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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