Anti-Calbindin antibody (ab156812)
Key features and details
- Goat polyclonal to Calbindin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
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Overview
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Product name
Anti-Calbindin antibody
See all Calbindin primary antibodies -
Description
Goat polyclonal to Calbindin -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Pig -
Immunogen
Synthetic peptide:
KTFVDQYGQRDDGK
by a Cysteine residue linker, corresponding to internal sequence amino acids 59-72 of Human Calbindin (NP_004920.1). -
Positive control
- Human Brain (Cerebellum) lysates, Mouse brain lysate, Rat brain lysate
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General notes
ab156812 overlaps EF hand 2, but not any calcium binding site.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab156812 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody notes
ab156812 overlaps EF hand 2, but not any calcium binding site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 30 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 30 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. -
Sequence similarities
Belongs to the calbindin family.
Contains 5 EF-hand domains. -
Domain
This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium. - Information by UniProt
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Database links
- Entrez Gene: 539930 Cow
- Entrez Gene: 611334 Dog
- Entrez Gene: 793 Human
- Entrez Gene: 12307 Mouse
- Entrez Gene: 100153645 Pig
- Entrez Gene: 83839 Rat
- Omim: 114050 Human
- SwissProt: P04467 Cow
see all -
Alternative names
- avian-type antibody
- CAB27 antibody
- CALB 1 antibody
see all
Images
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Anti-Calbindin antibody (ab156812) at 0.03 µg/ml + Human Brain Cerebellum lysate (in RIPA buffer). at 35 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted? -
All lanes : Anti-Calbindin antibody (ab156812) at 0.1 µg/ml
Lane 1 : Mouse brain tissue lysate
Lane 2 : rat brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 30 kDaPrimary incubation was 1 hour. Detected by chemiluminescence.
Datasheets and documents
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Datasheet download
References (1)
ab156812 has been referenced in 1 publication.
- Gebril H et al. Developmental Role of Adenosine Kinase in the Cerebellum. eNeuro 8:N/A (2021). PubMed: 33863781