Overview

  • Product name
  • Description
    Goat polyclonal to Calbindin
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    KTFVDQYGQRDDGK

    by a Cysteine residue linker, corresponding to internal sequence amino acids 59-72 of Human Calbindin (NP_004920.1).

  • Positive control
    • Human Brain (Cerebellum) lysates, Mouse brain lysate, Rat brain lysate
  • General notes


    ab156812 overlaps EF hand 2, but not any calcium binding site.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab156812 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notes
    ab156812 overlaps EF hand 2, but not any calcium binding site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 30 kDa).

Target

Images

  • Anti-Calbindin antibody (ab156812) at 0.03 µg/ml + Human Brain Cerebellum lysate (in RIPA buffer). at 35 µg

    Developed using the ECL technique.

    Predicted band size: 30 kDa
    Observed band size: 26 kDa (why is the actual band size different from the predicted?)

  • All lanes : Anti-Calbindin antibody (ab156812) at 0.1 µg/ml

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : rat brain tissue lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 30 kDa



    Primary incubation was 1 hour. Detected by chemiluminescence.

References

ab156812 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Cell (fresh mouse brain tissue)
Permeabilization
Yes - 0.5% Triton
Specification
fresh mouse brain tissue
Blocking step
NDS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Sharone Naor

Verified customer

Submitted Mar 14 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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