Recombinant
RabMAb

Anti-Calbindin antibody [EP3478] (ab108404)

Overview

  • Product name
    Anti-Calbindin antibody [EP3478]
    See all Calbindin primary antibodies
  • Description
    Rabbit monoclonal [EP3478] to Calbindin
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IHC-Frmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • Positive control
    • Fetal brain, fetal cerebellum, fetal hypothalamus and fetal pituitary lysates; Human brain tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab108404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
IHC-P 1/100 - 1/250.

Antigen retrieval is recommended.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100.
IHC-Fr 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
    • Sequence similarities
      Belongs to the calbindin family.
      Contains 5 EF-hand domains.
    • Domain
      This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.
    • Information by UniProt
    • Database links
    • Alternative names
      • avian-type antibody
      • CAB27 antibody
      • CALB 1 antibody
      • CALB antibody
      • CALB1 antibody
      • CALB1_HUMAN antibody
      • Calbindin 1 28kDa antibody
      • Calbindin antibody
      • Calbindin D28 antibody
      • D 28K antibody
      • D-28K antibody
      • D28K antibody
      • OTTHUMP00000166027 antibody
      • OTTHUMP00000225441 antibody
      • RTVL H protein antibody
      • Vitamin D dependent calcium binding protein antibody
      • Vitamin D dependent calcium binding protein avian type antibody
      • Vitamin D-dependent calcium-binding protein antibody
      see all

    Images

    • Immunohistochemical analysis of mouse frozen cerebellum tissue sections with ab108404 labelling Calbindin at 1/500. Sections were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).  The image shows specific staining on Purkinje cells of mouse cerebellum.

      Secondary antibody only was used as negative control and is shown in the inset.

    • ab108404 showing positive staining in Normal kidney tissue.

    • All lanes : Anti-Calbindin antibody [EP3478] (ab108404) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : Fetal cerebellum lysate
      Lane 3 : Fetal hypothalamus lysate
      Lane 4 : Fetal pituitary lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 30 kDa
    • Immunocytochemistry/Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma) cells labelling Calbindin with ab108404 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at dilution of 1/200. Nuclei were counterstained with DAPI (blue).

      Confocal image showing nuclear and cytoplasmic staining on Neuro2a cultured with 2% FBS.

    • ab108404, at 1/100 dilution, staining Calbindin in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • ab108404 showing negative staining in Normal heart tissue.

    • ab108404 showing negative staining in Normal colon tissue.

    • ab108404 showing negative staining in Normal liver tissue.

    References

    This product has been referenced in:
    • Dietrich P  et al. Elimination of huntingtin in the adult mouse leads to progressive behavioral deficits, bilateral thalamic calcification, and altered brain iron homeostasis. PLoS Genet 13:e1006846 (2017). Read more (PubMed: 28715425) »
    • McMahon SM  et al. Multiple cytosolic calcium buffers in posterior pituitary nerve terminals. J Gen Physiol 147:243-54 (2016). WB ; Rat . Read more (PubMed: 26880753) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Fukomys anselli Tissue sections (Retina)
    Permeabilization
    Yes - Triton X-100 in Blocking Solution
    Specification
    Retina
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Fixative
    Davidson's Fixative
    Username

    Abcam user community

    Verified customer

    Submitted Jul 28 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up