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Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse Calbindin.
(Peptide available as ab60452.)
Our Abpromise guarantee covers the use of ab49899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 30 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
Immunohistochemistical detection of Calbindin using antibody ab49899 on PFA perfusion-fixed frozen rat brain sections (30um). Antigen retrieval step: None Primary Antibody diluted at 1/300 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary Antibody: Goat anti-rabbit Alexa Fluor® 488 (1/1000). ab49899 was used on sections of rat brain in free floating IHC; expected staining was observed on rat brain. The submitted images show staining obtained in the cerebral cortex (A,B). The images were taken using the objective X5 (A) and X40 (B) and show the neuronal profiles (arrows) and neuronal processes.