Anti-Calcineurin A antibody [EP1669Y] (ab52761)

Overview

  • Product nameAnti-Calcineurin A antibody [EP1669Y]
    See all Calcineurin A primary antibodies
  • Description
    Rabbit monoclonal [EP1669Y] to Calcineurin A
  • SpecificityThis antibody reacts with Calcineurin A.
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human
  • Immunogen

    A synthetic peptide corresponding to residues on the N terminus of human Calcineurin A.

  • Positive control
    • WB: Hela cell lysate.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab52761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
IP 1/100.
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC or IHC-P.
  • Target

    • FunctionCalcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.
    • Sequence similaritiesBelongs to the PPP phosphatase family. PP-2B subfamily.
    • Cellular localizationNucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha isoform formerly PPP2B antibody
      • Calcineurin A alpha antibody
      • Calcineurin A1 antibody
      • CalcineurinA antibody
      • Calmodulin dependent calcineurin A subunit alpha isoform antibody
      • Calmodulin-dependent calcineurin A subunit alpha isoform antibody
      • CALN antibody
      • CALNA 1 antibody
      • CALNA antibody
      • CALNA1 antibody
      • CAM PRP catalytic subunit antibody
      • CAM-PRP catalytic subunit antibody
      • CCN 1 antibody
      • CCN1 antibody
      • CNA 1 antibody
      • CNA alpha antibody
      • CNA antibody
      • CNA1 antibody
      • PP2BA_HUMAN antibody
      • PPP2B antibody
      • Ppp3ca antibody
      • Protein phosphatase 2B catalytic subunit antibody
      • Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform antibody
      • Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA antibody
      • Protein phosphatase 3 catalytic subunit alpha isozyme antibody
      • Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform antibody
      • Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform antibody
      see all

    Anti-Calcineurin A antibody [EP1669Y] images



    • Predicted band size : 59 kDa
      Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

      Lane 1: Wild-type HAP1 cell lysate (20 µg)

      Lane 2: Calcineurin A knockout HAP1 cell lysate (20 µg)

      Lane 3: A431 cell lysate (20 µg)

      Lane 4: HeLa cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab52761 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab52761 was shown to specifically react with Calcineurin A when Calcineurin A knockout samples were used. Wild-type and Calcineurin A knockout samples were subjected to SDS-PAGE. ab52761 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Anti-Calcineurin A antibody [EP1669Y] (ab52761) at 1/10000 dilution + Hela cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 59 kDa
      Observed band size : 59 kDa
    • Overlay histogram showing HeLa cells stained with ab52761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52761, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References for Anti-Calcineurin A antibody [EP1669Y] (ab52761)

    ab52761 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (12)
    Sample Human Tissue lysate - whole (CNS tissue)
    Specification CNS tissue
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Submitted Aug 01 2014

    Application Western blot
    Loading amount 75 µg
    Gel Running Conditions Reduced Denaturing (15)
    Sample Mouse Tissue lysate - whole (brain Tissue)
    Specification brain Tissue
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted May 13 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Hamster Cell lysate - whole cell (CHO)
    Loading amount 5 µg
    Specification CHO
    Gel Running Conditions Reduced Denaturing (10-20% tris-glycine gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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    Verified customer

    Submitted Aug 13 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"