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Rabbit polyclonal to Calcineurin B
Recombinant fragment, corresponding to a region within amino acids 1-154 of Human Calcineurin B (P63098).
- NT2D1, IMR32, U-87 MG and MCF7 whole cell lysates; Human colon carcinoma tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 19 kDa.
||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
Belongs to the calcineurin regulatory subunit family.
Contains 4 EF-hand domains.
Information by UniProt
- alpha isoform (calcineurin B, type I) antibody
- calcineurin B, type I (19kDa) antibody
- Calcineurin subunit B type 1 antibody
Western blot - Anti-Calcineurin B antibody (ab126266)
Anti-Calcineurin B antibody (ab126266) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg
Predicted band size : 19 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B antibody (ab126266)
ab126266, at 1/250 dilution, staining Calcineurin B in paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"