Anti-Calcipressin 1 antibody (ab83271)

Overview

  • Product nameAnti-Calcipressin 1 antibody
    See all Calcipressin 1 primary antibodies
  • Description
    Rabbit polyclonal to Calcipressin 1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide: PVINYDLLYA ISKLGPGEKY ELHAATDTTP SVVVHVCESD QEKEEEEEME , corresponding to internal sequence amino acids 180-229 of Human Calcipressin 1 (NP_004405).

  • Positive control
    • Fetal muscle lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionInhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A. Could play a role during central nervous system development.
  • Tissue specificityHighly expressed heart, brain and skeletal muscle. Also expressed in all other tissues.
  • Sequence similaritiesBelongs to the RCAN family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adapt 78 antibody
    • Adapt78 antibody
    • Calcipressin 1 antibody
    • Calcipressin-1 antibody
    • Calcium and oxidant inducible mRNA antibody
    • CSP 1 antibody
    • CSP1 antibody
    • Down syndrome candidate region 1 antibody
    • Down syndrome critical region gene 1 antibody
    • Down syndrome critical region protein 1 antibody
    • DSC 1 antibody
    • DSC1 antibody
    • DSCR 1 antibody
    • DSCR1 antibody
    • MCIP 1 antibody
    • MCIP1 antibody
    • Modulatory calcineurin interacting protein 1 antibody
    • Myocyte enriched calcineurin interacting protein 1 antibody
    • Myocyte-enriched calcineurin-interacting protein 1 antibody
    • Near DSCR proline rich protein antibody
    • RCAN1 antibody
    • RCAN1_HUMAN antibody
    • RCN 1 antibody
    • RCN1 antibody
    • Regulator of calcineurin 1 antibody
    see all

Anti-Calcipressin 1 antibody images

  • Anti-Calcipressin 1 antibody (ab83271) at 1 µg/ml + Fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 23 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Calcipressin 1 antibody (ab83271) at 1/1000 dilution

    Lane 1 : Whole cell lysate prepared from primary mouse bone marrow derived osteoclasts with a point mutation in VATPase
    Lane 2 : Whole cell lysate prepared from wild type mouse osteoclasts

    Secondary
    HRP conjugated anti-rabbit at 1/2000 dilution

    Predicted band size : 23 kDa

References for Anti-Calcipressin 1 antibody (ab83271)

ab83271 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (cultured cortical neuron)
Loading amount 10 µg
Specification cultured cortical neuron
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Feb 25 2013

Application Western blot
Sample Mouse Cell lysate - other (cortical neurons)
Loading amount 10 µg
Specification cortical neurons
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Feb 08 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"