Anti-Calcitonin antibody (ab8553)
Key features and details
- Rabbit polyclonal to Calcitonin
- Suitable for: Sandwich ELISA, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Calcitonin antibody
See all Calcitonin primary antibodies -
Description
Rabbit polyclonal to Calcitonin -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISA, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Horse, Dog, Pig -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Medullary carcinoma of the thyroid or thyroid c-cell hyperplasia.
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General notes
Prolonged fixation in buffered formalin can destroy the epitope
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [16B5] to Calcitonin (ab11493).
For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab11493 as Capture. |
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IHC-P | (2) |
1/300. Antigen retrieval is not essential but may optimise staining.
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WB |
1/200 - 1/500. Predicted molecular weight: 15 kDa.
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Notes |
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Sandwich ELISA
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [16B5] to Calcitonin (ab11493). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab11493 as Capture. |
IHC-P
1/300. Antigen retrieval is not essential but may optimise staining. |
WB
1/200 - 1/500. Predicted molecular weight: 15 kDa. |
Target
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Relevance
Calcitonin is a 32 amino acid peptide hormone synthesized by the parafollicular cells of the thyroid. It causes a rapid, but short lived, reduction in serum calcium and phosphate by promoting the incorporation of those ions in the bones. This effect is opposite to that of parathyroid hormone. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity. -
Cellular localization
Cytoplasmic and Secreted -
Database links
- Entrez Gene: 403946 Dog
- Entrez Gene: 796 Human
- Entrez Gene: 12310 Mouse
- Entrez Gene: 24241 Rat
- Omim: 114130 Human
- SwissProt: P41547 Dog
- SwissProt: P01258 Human
- SwissProt: P70160 Mouse
see all -
Alternative names
- CALC 1 antibody
- CALC A antibody
- CALC1 antibody
see all
Images
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Standard Curve for Calcitonin dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [16B5] to Calcitonin (ab11493) at 5ug/ml and Detector Antibody Rabbit polyclonal to Calcitonin (ab8553) at 0.5ug/ml
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Anti-Calcitonin antibody (ab8553) at 1/25 dilution + Human thyroid lysate.
Predicted band size: 15 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemical analysis of human thyroid staining with ab8553.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab8553 has been referenced in 1 publication.
- Yerbury JJ et al. alpha2-Macroglobulin and haptoglobin suppress amyloid formation by interacting with prefibrillar protein species. J Biol Chem 284:4246-54 (2009). WB ; Human . PubMed: 19074141