Anti-Calcitonin antibody, prediluted (ab8548)


  • Product nameAnti-Calcitonin antibody, prediluted
    See all Calcitonin primary antibodies
  • Description
    Rabbit polyclonal to Calcitonin, prediluted
  • SpecificityThis antibody stains specifically for human parafollicular cells (C-cells) of normal and hyperplastic thyroid as well as medullary thyroid carcinoma. No staining of other cells or tissues has been observed.
  • Tested applicationsSuitable for: WB, IHC-FoFr, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Horse, Dog, Pig
  • Immunogen

    Synthetic human calcitonin 1-32.

  • Positive control
    • Medullary carcinoma of the thyroid or thyroid c-cell hyperplasia.
  • General notesThis antibody has been quality controled using formalin-fixed paraffin-embedded human thyroid tissue sections.



Our Abpromise guarantee covers the use of ab8548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 15 kDa.
IHC-FoFr Use at an assay dependent dilution.
The prediluted antibody can be used on frozen cryostat sections as well as formalin-fixed paraffin-embedded tissue sections. For paraffin-embedded tissue sections, we recommend an incubation time and temperature of 30 minutes at 37°C for this antibody. N.B Prolonged fixation in buffered formalin can destroy the epitope.
IHC-P Use at an assay dependent dilution.


  • RelevanceCalcitonin is a 32 amino acid peptide hormone synthesized by the parafollicular cells of the thyroid. It causes a rapid, but short lived, reduction in serum calcium and phosphate by promoting the incorporation of those ions in the bones. This effect is opposite to that of parathyroid hormone. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.
  • Cellular localizationCytoplasmic and Secreted
  • Database links
  • Alternative names
    • Alpha CGRP antibody
    • alpha type CGRP antibody
    • CALC 1 antibody
    • CALC A antibody
    • CALC1 antibody
    • calcitonin 1 antibody
    • calcitonin gene related peptide 1 antibody
    • calcitonin gene related peptide I antibody
    • calcitonin related polypeptide alpha antibody
    • CGRP 1 antibody
    • CGRP antibody
    • CGRP I antibody
    • CGRP1 antibody
    • CT antibody
    • katacalcin antibody
    • KC antibody
    see all

Anti-Calcitonin antibody, prediluted images

  • Anti-Calcitonin antibody, prediluted (ab8548) at 1/25 dilution + Human thyroid lysate

    Predicted band size : 15 kDa
    Observed band size : 14 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Calcitonin antibody, prediluted (ab8548)

ab8548 has not yet been referenced specifically in any publications.

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