Anti-Calcitonin receptor antibody (ab11042)

Overview

  • Product name
    Anti-Calcitonin receptor antibody
    See all Calcitonin receptor primary antibodies
  • Description
    Rabbit polyclonal to Calcitonin receptor
  • Specificity
    ab11042 recognises the calcitonin receptor in human, mouse and rat. The calcitonin receptor is a seven transmembrane domain cell surface protein which is coupled to G protein messenger systems. This antibody recognises an epitope within the cytoplasmic domain that is common to both C1a and C1b rat isoforms.
  • Tested applications
    Suitable for: IHC-Fr, IHC-FoFr, IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    KGLPIYICHQEPRNPPVSNN

    , corresponding to amino acids 476-496 of Rat Calcitonin receptor

  • Positive control
    • Kidney, cultered osteoclasts.
  • General notes
    Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/10 - 1/50.
IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/10 - 1/50. We recommend the use of 4% paraformaldehyde fixation, rather than formalin fixation. A sensitive detection system is also recommended. e.g. A catalysed signal amplification system. This product does not require protein digestion pre-treatment This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
WB Use at an assay dependent concentration. Predicted molecular weight: 56.9 kDa.
ELISA 1/10000.

Target

Images

  • ab11042 staining Calcitonin receptor in Rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton-X, blocked with 5% serum for 45 minutes at 22°C. The sample was incubated with primary antibody (1/500) at 4°C for 18 hours. An Alexa Fluor® 488-conjugated Donkey anti-rabbit polyclonal (1/500) was used as the secondary antibody

    See Abreview

References

This product has been referenced in:
  • Wu X  et al. IAPP modulates cellular autophagy, apoptosis, and extracellular matrix metabolism in human intervertebral disc cells. Cell Death Discov 3:16107 (2017). Read more (PubMed: 28149534) »
  • Bian Z  et al. Cd47-Sirpa interaction and IL-10 constrain inflammation-induced macrophage phagocytosis of healthy self-cells. Proc Natl Acad Sci U S A 113:E5434-43 (2016). Read more (PubMed: 27578867) »

See all 13 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Brain sections)
Specification
Brain sections
Permeabilization
Yes - 0.3% Triton-X 100
Fixative
Paraformaldehyde
Username

Mr. Vaibhav Konanur

Verified customer

Submitted Oct 28 2014


We have only two antibodies anti-human calcitonin receptor guaranteed to work in WB in our catalogue: ab103422 and ab11042.

Both of these should recognise all three human isoforms since there is a large sequence homology between all isof...

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Vielen Dank für Ihre Email.



Leider haben Sie Recht, was das austesten mit der technovit 9100neu angeht, und auch versenden wir keine Proben, da wir bei über 90.000Produkten gar nicht die logistischen Möglichkeiten habe...

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Thank you for your enquiry. Ab11042 was raised against a synthetic peptide with the following sequence: KGLPIYICHQEPRNPPVSNN-NH2. I have updated the datasheet with this information for future reference. I hope this information helps. Please do...

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I have just received the following information from the source of ab11042: "The cross-reactivity of this antibody with the human calcitonin receptor has been demonstrated by the antibody originator using human foetal kidney (pathology specimens), fi...

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I'm sorry to hear you are having problem with ab11042. I would like to suggest trying the immunohistochemistry steps without antigen retrieval prior to staining of paraffin embedded sections. I would also recommend using triton (0.3%v/v)in PBS in ...

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Thank you for your enquiry. This antibody is specific for the calcitonin receptor. This receptor is expressed by osteoclasts but expression has also been reported in the kidney, central and peripheral nervous systems. We hope that this information ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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