Calcium Assay Kit (Colorimetric) (ab102505)
Key features and details
- Assay type: Quantitative
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.1 mM
Overview
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Product name
Calcium Assay Kit (Colorimetric)
See all Calcium kits -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate -
Assay type
Quantitative -
Sensitivity
> 0.1 mM -
Range
0.1 mM - 25 mM -
Assay time
0h 20m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Calcium Assay Kit (Colorimetric) ab102505 provides a simple assay to determine calcium concentration within the physiological range of 0.4 – 100 mg/dL (0.1 – 25 mM).
In the calcium assay protocol, a chromogenic complex is formed between calcium ions and 0-cresolphthalein. The complex is measured at OD = 575 nm.
This calcium assay detects free calcium ions only.
Calcium assay protocol summary:
- add samples and standards to wells
- add chromogenic reagent, add calcium assay buffer and incubate for 5-10 min at room temp
- analyze with microplate reader -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K380 Calcium Colorimetric Assay Kit. K380-250 is the same size as the 250 test size of ab102505.
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Platform
Microplate reader
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 250 tests Calcium Assay Buffer 1 x 15ml Calcium Standard 1 x 100µl Chromogenic Reagent 1 x 25ml -
Research areas
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Relevance
Calcium is essential for all living organisms, where Ca2+ sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% of calcium is found in bones and teeth with the remaining 1% found in the blood and soft tissue. Serum calcium levels are tightly controlled (8.4-11.4 mg/dL) and any variation outside this range can have serious effects. Calcium plays a role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism. -
Alternative names
- Ca++
- Ca2+
Associated products
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Corresponding kit
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Related Products
Images
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Time course of mineralization. Measurement of LDH activity. After a 15-day cultivation period of each of 2 x 106 osteoblasts growing in OSC-supplemented media (OSC+) and control osteoblasts growing in non-OSC-supplemented media (OSC-) the LDH activity in the cell free supernatants was determined as described in Material and Methods. Osteoblasts treated with 1% (v/v) Triton-X100 for 1 hour (c) served as a second control. The experiments were carried out with three primary cultures in triplicate
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Effect of PFOA on calcium concentrations in cultured human osteoblasts after exposure for 3 or 5 weeks. Plots represent mean ± SD.
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Ca2+ concentration (millimolar) in various biologicals. Shown bars are average of values from dilutions neat to 1:8.
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Example of standard curve obtained using ab102505
Datasheets and documents
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SDS download
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Datasheet download
References (106)
ab102505 has been referenced in 106 publications.
- Phan K et al. Multiple pathways of lipid dysregulation in amyotrophic lateral sclerosis. Brain Commun 5:fcac340 (2023). PubMed: 36632187
- Yadav MK et al. Lentiviral vector mediated gene therapy for type I Dent disease ameliorates Dent disease-like phenotypes for three months in ClC-5 null mice. Mol Ther Methods Clin Dev 27:149-166 (2022). PubMed: 36284768
- Martino E et al. Milk Exosomal miR-27b Worsen Endoplasmic Reticulum Stress Mediated Colorectal Cancer Cell Death. Nutrients 14:N/A (2022). PubMed: 36501111
- Chao CT et al. Deep Learning-Assisted Repurposing of Plant Compounds for Treating Vascular Calcification: An In Silico Study with Experimental Validation. Oxid Med Cell Longev 2022:4378413 (2022). PubMed: 35035662
- Shi LL et al. Liraglutide Alleviates Diabetic Atherosclerosis through Regulating Calcification of Vascular Smooth Muscle Cells. Dis Markers 2022:5013622 (2022). PubMed: 35510038