Anti-Calcium-independent Phospholipase A2 antibody (ab103258)

Overview

  • Product nameAnti-Calcium-independent Phospholipase A2 antibody
    See all Calcium-independent Phospholipase A2 primary antibodies
  • Description
    Rabbit polyclonal to Calcium-independent Phospholipase A2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 558-588 of Human Calcium-independent Phospholipase A2.

  • Positive control
    • HepG2 cell lysate; Human testis carcinoma

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityAmmonium Sulphate Precipitation
  • Purification notesab103258 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 90 kDa.Can be blocked with Human Calcium-independent Phospholipase A2 peptide (ab205543).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionCatalyzes the release of fatty acids from phospholipids. It has been implicated in normal phospholipid remodeling, nitric oxide-induced or vasopressin-induced arachidonic acid release and in leukotriene and prostaglandin production. May participate in fas mediated apoptosis and in regulating transmembrane ion flux in glucose-stimulated B-cells. Has a role in cardiolipin (CL) deacylation. Required for both speed and directionality of monocyte MCP1/CCL2-induced chemotaxis through regulation of F-actin polymerization at the pseudopods.
    Isoform ankyrin-iPLA2-1 and isoform ankyrin-iPLA2-2, which lack the catalytic domain, are probably involved in the negative regulation of iPLA2 activity.
  • Tissue specificityFour different transcripts were found to be expressed in a distinct tissue distribution.
  • Involvement in diseaseNeurodegeneration with brain iron accumulation 2B
    Neurodegeneration with brain iron accumulation 2A
    Parkinson disease 14
  • Sequence similaritiesContains 7 ANK repeats.
    Contains 1 patatin domain.
  • Cellular localizationCytoplasm and Membrane. Recruited to the membrane-enriched pseudopod upon MCP1/CCL2 stimulation in monocytes.
  • Information by UniProt
  • Database links
  • FormThe isoform LH-iPLA2 is located at the membrane. The isoform SH-iPLA2 is cytoplasmic.
  • Alternative names
    • 85 kDa calcium independent phospholipase A2 antibody
    • 85/88 kDa calcium-independent phospholipase A2 antibody
    • BB112799 antibody
    • CaI PLA2 antibody
    • CaI-PLA2 antibody
    • CaIPLA2 antibody
    • Calcium independent phospholipase A2 antibody
    • CTA-228A.2 antibody
    • Cytosolic calcium independent phospholipase A2 antibody
    • EC 3.1.1.4 antibody
    • Group VI phospholipase A2 antibody
    • Group VI, A antibody
    • GVI antibody
    • GVI PLA2 antibody
    • INAD1 antibody
    • Intracellular membrane associated calcium independent phospholipase A2 beta antibody
    • Intracellular membrane-associated calcium-independent phospholipase A2 beta antibody
    • iPLA(2)beta antibody
    • iPLA2 beta antibody
    • iPLA2-beta antibody
    • IPLA2-VIA antibody
    • iPLA2beta antibody
    • NBIA2 antibody
    • NBIA2A antibody
    • NBIA2B antibody
    • Neurodegeneration with brain iron accumulation 2 antibody
    • OTTHUMP00000199457 antibody
    • PARK14 antibody
    • Patatin like phospholipase domain containing 9 antibody
    • Patatin like phospholipase domain containing protein 9 antibody
    • Patatin-like phospholipase domain-containing protein 9 antibody
    • Phospholipase A2 calcium independent antibody
    • Phospholipase A2 calcium independent group VI A antibody
    • Phospholipase A2 group VI antibody
    • Phospholipase A2 group VI cytosolic calcium independent antibody
    • Phospholipase A2, group VI (cytosolic, calcium independent) antibody
    • PLA2 antibody
    • PLA2G6 antibody
    • PLPL9_HUMAN antibody
    • PNPLA9 antibody
    see all

Anti-Calcium-independent Phospholipase A2 antibody images

  • Anti-Calcium-independent Phospholipase A2 antibody (ab103258) at 1/50 dilution + HepG2 cell lysate at 35 µg

    Predicted band size : 90 kDa
  • ab103258, at 1/50 dilution, staining Calcium-independent Phospholipase A2 in formalin-fixed, paraffin-embedded Human testis carcinoma by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.

References for Anti-Calcium-independent Phospholipase A2 antibody (ab103258)

ab103258 has not yet been referenced specifically in any publications.

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