Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody (ab104416)
Key features and details
- Rabbit polyclonal to Calcium-independent Phospholipase A2/PLA2G6
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody
See all Calcium-independent Phospholipase A2/PLA2G6 primary antibodies -
Description
Rabbit polyclonal to Calcium-independent Phospholipase A2/PLA2G6 -
Host species
Rabbit -
Specificity
Reactive against mammalian transfected lysate. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein within Human Calcium-independent Phospholipase A2/PLA2G6. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_001004426.1 -
Positive control
- Human kidney tissue lysate, Mouse kidney tissue lysate, Transfected 293T cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab104416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 90 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 90 kDa. |
Target
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Function
Catalyzes the release of fatty acids from phospholipids. It has been implicated in normal phospholipid remodeling, nitric oxide-induced or vasopressin-induced arachidonic acid release and in leukotriene and prostaglandin production. May participate in fas mediated apoptosis and in regulating transmembrane ion flux in glucose-stimulated B-cells. Has a role in cardiolipin (CL) deacylation. Required for both speed and directionality of monocyte MCP1/CCL2-induced chemotaxis through regulation of F-actin polymerization at the pseudopods.
Isoform ankyrin-iPLA2-1 and isoform ankyrin-iPLA2-2, which lack the catalytic domain, are probably involved in the negative regulation of iPLA2 activity. -
Tissue specificity
Four different transcripts were found to be expressed in a distinct tissue distribution. -
Involvement in disease
Neurodegeneration with brain iron accumulation 2B
Neurodegeneration with brain iron accumulation 2A
Parkinson disease 14 -
Sequence similarities
Contains 7 ANK repeats.
Contains 1 patatin domain. -
Cellular localization
Cytoplasm and Membrane. Recruited to the membrane-enriched pseudopod upon MCP1/CCL2 stimulation in monocytes. - Information by UniProt
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Database links
- Entrez Gene: 8398 Human
- Entrez Gene: 53357 Mouse
- Omim: 603604 Human
- SwissProt: O60733 Human
- SwissProt: P97819 Mouse
- Unigene: 170479 Human
- Unigene: 155620 Mouse
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Form
The isoform LH-iPLA2 is located at the membrane. The isoform SH-iPLA2 is cytoplasmic. -
Alternative names
- 85 kDa calcium independent phospholipase A2 antibody
- 85/88 kDa calcium-independent phospholipase A2 antibody
- BB112799 antibody
see all
Images
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Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody (ab104416) at 1 µg/ml + Human kidney tissue lysate at 50 µg
Predicted band size: 90 kDa -
Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody (ab104416) at 1 µg/ml + Mouse kidney tissue lysate at 50 µg
Predicted band size: 90 kDa -
All lanes : Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody (ab104416) at 1 µg/ml
Lane 1 : Calcium-independent Phospholipase A2/PLA2G6 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 90 kDa
Observed band size: 84 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (0)
ab104416 has not yet been referenced specifically in any publications.