Anti-Calcium Pump PMCA3 ATPase antibody (ab84521)

Overview

  • Product nameAnti-Calcium Pump PMCA3 ATPase antibody
    See all Calcium Pump PMCA3 ATPase primary antibodies
  • Description
    Rabbit polyclonal to Calcium Pump PMCA3 ATPase
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (GDMANSSIEF HPKPQQQRDV PQAGGFGCTL AELRTLMELR GAEALQKIEE) of Human Calcium Pump PMCA3 ATPase (NP_001001344).

  • Positive control
    • Human fetal heart lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 134 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceCalcium Pump PMCA3 ATPase belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. Calcium Pump PMCA3 ATPase is the plasma membrane calcium ATPase isoform 3.
  • Cellular localizationCell membrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • ATP2B3 antibody
    • ATP2B3 protein antibody
    • ATPase Ca++ transporting plasma membrane 3 antibody
    • Plasma membrane calcium ATPase 3 antibody
    • Plasma membrane calcium ATPase isoform 3 antibody
    • Plasma membrane calcium pump isoform 3 antibody
    • Plasma membrane calcium transporting ATPase 3 antibody
    • PMCA 3 antibody
    • PMCA3 antibody
    • PMCA3a antibody
    see all

Anti-Calcium Pump PMCA3 ATPase antibody images

  • Anti-Calcium Pump PMCA3 ATPase antibody (ab84521) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 134 kDa
    Observed band size : 115 kDa (why is the actual band size different from the predicted?)

References for Anti-Calcium Pump PMCA3 ATPase antibody (ab84521)

ab84521 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"