Anti-Calcium Sensing Receptor [5C10, ADD] antibody (ab19347)

Overview

  • Product nameAnti-Calcium Sensing Receptor [5C10, ADD] antibodySee all Calcium Sensing Receptor primary antibodies ...
  • Description
    Mouse monoclonal [5C10, ADD] to Calcium Sensing Receptor
  • Tested applicationsIHC-P, ICC/IF, IP, Flow Cyt, WB, IHC-FoFr, ELISA, IHC-Fr more details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Pig
  • Immunogen

    Synthetic peptide: ADDDYGRPGIEKFREEAEERDI, corresponding to amino acids 214-235 of Human Calcium Sensing Receptor.

  • Positive control
    • Detects human, bovine and rat calcium sensing receptor.

Properties

Applications

Our Abpromise guarantee covers the use of ab19347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 130, 150 kDa.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19056349
ELISA Use at an assay dependent concentration.
IHC-Fr Use a concentration of 2 µg/ml.

Target

  • FunctionSenses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system.
  • Tissue specificityExpressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta.
  • Involvement in diseaseDefects in CASR are the cause of familial hypocalciuric hypercalcemia type 1 (FHH) [MIM:145980]. FHH is characterized by altered calcium homeostasis. Affected individuals exhibit mild or modest hypercalcemia, relative hypocalciuria, and inappropriately normal PTH levels.
    Defects in CASR are the cause of neonatal severe primary hyperparathyroidism (NSHPT) [MIM:239200]. NSHPT is a rare autosomal recessive life-threatening disorder characterized by very high serum calcium concentrations, skeletal demineralization, and parathyroid hyperplasia. In some instances NSHPT has been demonstrated to be the homozygous form of FHH.
    Defects in CASR are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. An autosomal recessive form of FIH also exists.
    Defects in CASR are the cause of idiopathic generalized epilepsy type 8 (IGE8) [MIM:612899]; also known as EIG8. A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Seizure types are variable, but include myoclonic seizures, absence seizures, febrile seizures, complex partial seizures, and generalized tonic-clonic seizures.
    Note=Homozygous defects in CASR can be a cause of primary hyperparathyroidism in adulthood. Patients suffer from osteoporosis and renal calculi, have marked hypercalcemia and increased serum PTH concentrations.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 3 family.
  • Post-translational
    modifications
    N-glycosylated.
    Ubiquitinated by RNF19A; which induces proteasomal degradation.
  • Cellular localizationCell membrane.
  • Target information above from: UniProt accession P41180 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Ca sensing receptor antibody
    • Ca2+ sensing receptor 1 antibody
    • Ca2+ sensing receptor antibody
    • CAR antibody
    • CaSR antibody
    • CASR_HUMAN antibody
    • EIG8 antibody
    • Extracellular calcium sensing receptor antibody
    • Extracellular calcium sensing receptor [Precursor] antibody
    • Extracellular calcium-sensing receptor [Precursor] antibody
    • Extracellular calcium-sensing receptor antibody
    • FHH antibody
    • FIH antibody
    • GPRC2A antibody
    • HHC antibody
    • HHC1 antibody
    • Hypocalciuric hypercalcemia 1 antibody
    • Hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism antibody
    • MGC138441 antibody
    • NSHPT antibody
    • Parathyroid Ca(2+) sensing receptor 1 antibody
    • Parathyroid Cell calcium sensing receptor antibody
    • Parathyroid Cell calcium-sensing receptor antibody
    • PCAR 1 antibody
    • PCAR1 antibody
    see all

Anti-Calcium Sensing Receptor [5C10, ADD] antibody images

  • Overlay histogram showing SH-SY5Y cells stained with ab19347 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19347, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed SH-SY5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • ab19347 at 1/100 staining rat brain (cerebral cortex) tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum before incubation with the primary antibody for 24 hours at 4°C. A biotinylated horse anti-mouse IgG was used as the secondary.

    See Abreview

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Calcium Sensing Receptor ab19347 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Calcium Sensing Receptor ab19347 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

References for Anti-Calcium Sensing Receptor [5C10, ADD] antibody (ab19347)

This product has been referenced in:
  • Hénaut L  et al. Calcimimetics increase CaSR expression and reduce mineralization in vascular smooth muscle cells: mechanisms of action. Cardiovasc Res 101:256-65 (2014). WB ; Human . Read more (PubMed: 24217682) »
  • Ding H  et al. Localization of CaSR antagonists in CaSR-expressing medullary thyroid cancer. J Clin Endocrinol Metab 98:E1722-9 (2013). Read more (PubMed: 24030941) »

See all 9 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step 1% BSA + 5% NGS as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Sample Human Tissue sections (blood vessels)
Specification blood vessels
Permeabilization No
Fixative Paraformaldehyde
Username

Mr. Florian Guilbaud

Verified customer

Submitted Mar 24 2014

Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
I am attaching ...

Read More

Thank you for your emails and telephone calls today.

I am very pleased to hear you would like to accept our offer and test ab19347 in IHC-P. I have arranged the following discount code:

DISCOUNT CODE: #####
Expiration date: #...

Read More

Thank you for your telephone enquiry earlier this morning.

As discussed on the telephone, I am sorry to conrirm that to our knowledge, ab19347 has not been tested in WB. Therefore, I can offer a discount off a future purchase if you buy ab19...

Read More

DISCOUNT CODE: xxx for ab19347
DISCOUNT CODE: xxx for ab71842
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab19347 and ab71842in chicken and/or IHC-Fr. Each code will give you: 1 free P...

Read More

Thank you for contacting us.

The catalogue number of blocking peptide is ab18608.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our produ...

Read More

Thank you for your enquiry and your interest in our products.

I can confirm it for you that this antibody (ab19347: Anti-Calcium Sensing Receptor antibody) has been tested for cross-reaction with rat, cow, human. Based on blast search (using ...

Read More

Thank you for contacting us again. I am very pleased to hear you are choosing to test ab18200 with your mouse samples. I have generated the discount code, this will entitle you to one free primary antibody before the expiration date: DISCOUNT C...

Read More

DISCOUNT CODE: ******* Expiration date: ******* I am very pleased to hear you would like to accept our offer and test ab19347 in mouse tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please subm...

Read More

Thank you for contacting us. The amino acid 374 -391 falls more towards N-terminal end of the protein; this sequence is present in the extracellular domain which extends from amino acid 20 to 612 (http://www.uniprot.org/uniprot/P41180). The sequenc...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"