Purification notesab84862 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filter serilised through a 0.22 µm membrane.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Predicted molecular weight: 52 kDa.
Use at an assay dependent dilution.
FunctionMay play a role in ruffle formation and actin cytoskeleton organization. Seems to negatively regulate constitutive secretion.
Tissue specificityExpressed in all tissues tested with highest expression in skeletal muscle and lowest in brain.
Cellular localizationCytoplasm > perinuclear region. Golgi apparatus. Cytoplasm > cytoskeleton. According to PubMed:7540613, localizes to nuclear dots. According to PubMed:9230084 and PubMed:12869526, it is not a nuclear dot-associated protein but localizes predominantly in the cytoplasm with a coarse-grained distribution preferentially close to the nucleus.