Overview

  • Product nameAnti-CALCOCO2 antibody
    See all CALCOCO2 primary antibodies
  • Description
    Rabbit polyclonal to CALCOCO2
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human CALCOCO2.

    (Peptide available as ab68587.)

  • Positive control
    • This antibody gave a positive signal in the following Human Lysates: TE 671 Whole Cell, HeLa Whole Cell - Hydroxyurea Treated (48hr, 2µM), Ramos Whole Cell, Placenta Tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab68588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
IP Use at an assay dependent concentration. PubMed: 23209807

Target

  • FunctionMay play a role in ruffle formation and actin cytoskeleton organization. Seems to negatively regulate constitutive secretion.
  • Tissue specificityExpressed in all tissues tested with highest expression in skeletal muscle and lowest in brain.
  • Cellular localizationCytoplasm > perinuclear region. Golgi apparatus. Cytoplasm > cytoskeleton. According to PubMed:7540613, localizes to nuclear dots. According to PubMed:9230084 and PubMed:12869526, it is not a nuclear dot-associated protein but localizes predominantly in the cytoplasm with a coarse-grained distribution preferentially close to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen nuclear dot 52 kDa protein antibody
    • CACO2_HUMAN antibody
    • Calcium binding and coiled coil domain 2 antibody
    • Calcium binding and coiled coil domain containing protein 2 antibody
    • Calcium-binding and coiled-coil domain-containing protein 2 antibody
    • CALCOCO 2 antibody
    • CALCOCO2 antibody
    • MGC17318 antibody
    • NDP52 antibody
    • Nuclear domain 10 protein 52 antibody
    • Nuclear domain 10 protein antibody
    • Nuclear domain 10 protein NDP52 antibody
    • Nuclear domain protein 52 antibody
    • Nuclear dot protein 52 antibody
    • OTTHUMP00000218344 antibody
    • OTTHUMP00000218345 antibody
    • OTTHUMP00000218346 antibody
    • OTTHUMP00000218347 antibody
    • OTTHUMP00000218349 antibody
    • OTTHUMP00000218351 antibody
    see all

Anti-CALCOCO2 antibody images

  • ab68588 stained HeLa cells. The cells were 4% PFA fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68588 at 1µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was  used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-CALCOCO2 antibody (ab68588) at 1 µg/ml

    Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 2 : HeLa Whole Cell Lysate - Hydroxyurea Treated (48hr, 2µM)
    Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 4 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes
  • IHC image of CALCOCO2 staining in Human Cerebral Cortex FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68588, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

References for Anti-CALCOCO2 antibody (ab68588)

This product has been referenced in:
  • Jo C  et al. Nrf2 reduces levels of phosphorylated tau protein by inducing autophagy adaptor protein NDP52. Nat Commun 5:3496 (2014). Read more (PubMed: 24667209) »
  • Baxt LA & Goldberg MB Host and bacterial proteins that repress recruitment of LC3 to Shigella early during infection. PLoS One 9:e94653 (2014). ICC/IF . Read more (PubMed: 24722587) »

See all 9 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rabbit Cell (smooth muscle cells)
Specification smooth muscle cells
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100 0,1%
Blocking step Gelatin from cold water fish skin as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Apr 08 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"