Anti-Caldesmon (phospho T753) antibody [EPR2211Y] (ab108356)

Overview

  • Product nameAnti-Caldesmon (phospho T753) antibody [EPR2211Y]
    See all Caldesmon primary antibodies
  • Description
    Rabbit monoclonal [EPR2211Y] to Caldesmon (phospho T753)
  • Tested applicationsSuitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding threonine 753 of Human Caldesmon.

  • Positive control
    • HeLa cell lysates treated with Calyculin A
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab108356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. MWt: smooth muscle 120-150 kDa, Non Muscle 70-80 kDa.
ICC 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • FunctionActin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.
    • Tissue specificityHigh-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
    • Sequence similaritiesBelongs to the caldesmon family.
    • DomainThe N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form.
    • Post-translational
      modifications
      In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization.
    • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > myofibril. On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).
    • Information by UniProt
    • Database links
    • Alternative names
      • CAD antibody
      • CALD 1 antibody
      • CALD1 antibody
      • CALD1_HUMAN antibody
      • Caldesmon 1 antibody
      • Caldesmon 1 Isoform 1 antibody
      • Caldesmon 1 Isoform 2 antibody
      • Caldesmon 1 Isoform 3 antibody
      • Caldesmon 1 Isoform 4 antibody
      • Caldesmon 1 Isoform 5 antibody
      • Caldesmon antibody
      • Caldesmon1 antibody
      • CDM antibody
      • H CAD antibody
      • HCAD antibody
      • L CAD antibody
      • LCAD antibody
      • MGC21352 antibody
      • NAG22 antibody
      see all

    Anti-Caldesmon (phospho T753) antibody [EPR2211Y] images

    • All lanes : Anti-Caldesmon (phospho T753) antibody [EPR2211Y] (ab108356) at 1/1000 dilution

      Lane 1 : Hela cell lysate, untreated
      Lane 2 : Hela cell lysate, treated with Calyculin A

      Lysates/proteins at 10 µg per lane.

    References for Anti-Caldesmon (phospho T753) antibody [EPR2211Y] (ab108356)

    ab108356 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"