Overview

  • Product nameAnti-CALHM2 antibody
  • Description
    Rabbit polyclonal to CALHM2
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    amino acids 124-185 (RGEAYVCALS EFVDPSSLTA REEHFPSAHA TEILARFPCK ENPDNLSDFR EEVSRRLRYE SQ) of Human CALHM2 (Q9HA72).

  • Positive control
    • Human colon tissue; RT-4 and U-251 MG cell lysates; Human Plasma, Liver and Tonsil lysates

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Anti-CALHM2 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in mitochondria. Recommended concentration of ab121446 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121446, at 1/400 dilution, staining CALHM2 in paraffin-embedded Human colon tissue by Immunohistochemistry.
  • All lanes : Anti-CALHM2 antibody (ab121446) at 1/250 dilution

    Lane 1 : RT-4 cell lysate.
    Lane 2 : U-251 MG cell lysate.
    Lane 3 : Human Plasma
    Lane 4 : Human liver lysate.
    Lane 5 : Human tonsil lysate.

    Developed using the ECL technique

References for Anti-CALHM2 antibody (ab121446)

ab121446 has not yet been referenced specifically in any publications.

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Thank you for contacting us with your question and for your interest in ab121446.

We only have full size vials in stock, so we don't have a small aliquot to send out for testing. However, for cases like this when an antibody has not been test...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"