Overview

  • Product nameAnti-CALM2 antibody
  • Description
    Mouse monoclonal to CALM2
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-150 of Human CALM2

Properties

Applications

Our Abpromise guarantee covers the use of ab54921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
  • Application notesICC/IF: Use at a concentration of 10 µg/ml.
    WB: Use at a concentration of 1 - 5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCalmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
    • Sequence similaritiesBelongs to the calmodulin family.
      Contains 4 EF-hand domains.
    • Post-translational
      modifications
      Ubiquitination results in a strongly decreased activity.
      Phosphorylation results in a decreased activity.
    • Cellular localizationCytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
    • Information by UniProt
    • Database links
    • Alternative names
      • CALM_HUMAN antibody
      • Calm3 antibody
      • Calmodulin 2 antibody
      • Calmodulin antibody
      • CaM antibody
      • CAMII antibody
      • PHKD antibody
      • PHKD2 antibody
      see all

    Anti-CALM2 antibody images

    • Western blot against tagged recombinant protein immunogen using ab54921 CALM2 antibody at 1ug/ml. Predicted band size of immunogen is 42 kDa.

      This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    • ab54921 at 10 ug/ml staining CALM2 in human Hela cells by Immunocytochemistry / Immunofluorescence.

    • IHC-P image of CALM2 (ab54921) on Skin cancer sections. The sections were fixed in Formaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 0.3% H2O2 solution for 10 mins at 22°C.

      See Abreview

    References for Anti-CALM2 antibody (ab54921)

    ab54921 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Skin, cancer)
    Specification Skin, cancer
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Novacastra pH 6
    Permeabilization No
    Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: 22°C
    Username

    Dr. Aamir Ahmed

    Verified customer

    Submitted Oct 15 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"