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Recombinant fragment within Human Calmegin. The exact sequence is proprietary.
Database link: O14967
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab172477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 93 kDa (predicted molecular weight: 70 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P image of Calmegin staining with ab172477 on tissue sections from adult marmoset testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab172477 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.
Immunohistochemical analysis of paraffin embedded Human testis tissue labeling Calmegin with ab172477 at 1/250 dilution.
Immunofluorescent analysis of Jurkat cells labeling Calmegin with ab172477 at 1/100 dilution.
Flow cytometric analysis of permeabilized Jurkat cells labeling Calmegin with ab172477 at 1/10 dilution (red) compared with a rabbit IgG (negative) (green).
Western blot analysis on immunoprecipitation pellet from Human testis lysate labeling Calmegin with ab172477 at 1/10 dilution.
ab172477 has not yet been referenced specifically in any publications.