Recombinant
RabMAb

Anti-Calmegin antibody [EPR11832-11] (ab172477)

Overview

  • Product name
    Anti-Calmegin antibody [EPR11832-11]
    See all Calmegin primary antibodies
  • Description
    Rabbit monoclonal [EPR11832-11] to Calmegin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Chimpanzee, Common marmoset
  • Immunogen

    Recombinant fragment within Human Calmegin. The exact sequence is proprietary.
    Database link: O14967

  • Positive control
    • Human testis, Mouse testis and Rat testis tissue lysate and Jurkat cell lysate; Human testis tissue; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab172477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 93 kDa (predicted molecular weight: 70 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • All lanes : Anti-Calmegin antibody [EPR11832-11] (ab172477) at 1/10000 dilution

    Lane 1 : Human testis tissue lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Mouse testis tissue lysate
    Lane 4 : Rat testis tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 70 kDa

  • IHC-P image of Calmegin staining with ab172477 on tissue sections from adult marmoset testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab172477 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.

    See Abreview

  • Immunohistochemical analysis of paraffin embedded Human testis tissue labeling Calmegin with ab172477 at 1/250 dilution.

  • Immunofluorescent analysis of Jurkat cells labeling Calmegin with ab172477 at 1/100 dilution.

  • Flow cytometric analysis of permeabilized Jurkat cells labeling Calmegin with ab172477 at 1/10 dilution (red) compared with a rabbit IgG (negative) (green).

  • Western blot analysis on immunoprecipitation pellet from Human testis lysate labeling Calmegin with ab172477 at 1/10 dilution.

References

ab172477 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Sample
Chimpanzee Tissue sections (adult testis)
Specification
adult testis
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. 煜欽 林

Verified customer

Submitted Nov 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Sample
Marmoset (common) Tissue sections (adult testis)
Specification
adult testis
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. 煜欽 林

Verified customer

Submitted Aug 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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