Overview

  • Product nameCalmidazolium chloride
  • Description
    Calmodulin inhibitor
  • Alternative names
    • R 24571
  • Biological descriptionCalmodulin inhibitor (IC50 = 10 nM). Elevates intracellular calcium, independent of calmodulin inhibition. Additionally inhibits adenylyl cylase activity.
  • Purity> 98%

Properties

  • Chemical name3-[Bis(4-chlorophenyl)methyl]-1-[2-(2,4-dichlorophenyl)-2-[(2,4-dichlorophenyl)methoxy)ethyl]-1H-imidazolium chloride
  • Molecular weight687.70
  • Chemical structureChemical Structure
  • Molecular formulaC31H23Cl7N2O
  • CAS Number57265-65-3
  • Storage instructionsStore at -20°C. The product can be stored for up to 12 months.
  • Solubility overviewSoluble in ethanol to 100 mM and in DMSO to 100 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

  • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab120658 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Calmidazolium chloride images

    • ab48394 staining LC3B (LC3-II) in HeLa cells treated with calmidazolium chloride (ab120658), by ICC/IF. Increase of LC3B (LC3-II) expression correlates with increased concentration of calmidazolium chloride, as described in literature.
      The cells were incubated at 37°C for 6h in media containing different concentrations of ab120658 (calmidazolium chloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab48394 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for Calmidazolium chloride (ab120658)

    This product has been referenced in:
    • Peppiatt CM  et al. Calmidazolium and arachidonate activate a calcium entry pathway that is distinct from store-operated calcium influx in HeLa cells. Biochem J 381:929-39 (2004). Read more (PubMed: 15130089) »
    • Shi J  et al. Multiple regulation by calcium of murine homologues of transient receptor potential proteins TRPC6 and TRPC7 expressed in HEK293 cells. J Physiol 561:415-32 (2004). Read more (PubMed: 15579537) »
    • Haunsø A  et al. Small ligands modulating the activity of mammalian adenylyl cyclases: a novel mode of inhibition by calmidazolium. Mol Pharmacol 63:624-31 (2003). Read more (PubMed: 12606770) »

    See 0 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab120658.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"