Overview

  • Product name
  • Description
    Rabbit polyclonal to CALML5
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: GELTPEEEAQ YKKAFSAVDT DGNGTINAQE LGAALKATGK NLSEAQLRKL ISEVDGDGDG EISFQEFLTA, corresponding to N terminal amino acids 3-72 of Human CALML5.

  • Positive control
    • Human skin tissue. CALML5 over-expression lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab122665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function
    Binds calcium. May be involved in terminal differentiation of keratinocytes.
  • Tissue specificity
    Particularly abundant in the epidermis where its expression is directly related to keratinocyte differentiation. Very low expression in lung.
  • Sequence similarities
    Contains 4 EF-hand domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • CALL5_HUMAN antibody
    • CALML5 antibody
    • Calmodulin like 5 antibody
    • Calmodulin-like protein 5 antibody
    • Calmodulin-like skin protein antibody
    • CLSP antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab122665 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122665 at 1/2500 dilution staining CALML5 in paraffin-embedded Human skin tissue by Immunohistochemistry.
  • All lanes : Anti-CALML5 antibody (ab122665) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : CALML5 over-expression lysate; co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

    Developed using the ECL technique

References

ab122665 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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