Overview

  • Product name
    Anti-Calmodulin 1/2/3 antibody
    See all Calmodulin 1/2/3 primary antibodies
  • Description
    Rabbit polyclonal to Calmodulin 1/2/3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Chicken, Cow, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Calmodulin 1/2/3 aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab123437)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Ramos; NIH3T3.

Properties

Applications

Our Abpromise guarantee covers the use of ab105498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).

Target

  • Relevance
    Function: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
  • Cellular localization
    Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
  • Database links
  • Form
    There are three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase.
  • Alternative names
    • CALM 1 antibody
    • CALM 2 antibody
    • CALM 3 antibody
    • CALM antibody
    • CALM1 antibody
    • CALM2 antibody
    • CALM3 antibody
    • CALML2 antibody
    • calmodulin 1 (phosphorylase kinase, delta) antibody
    • Calmodulin 1 antibody
    • Calmodulin 2 (phosphorylase kinase, delta) antibody
    • Calmodulin 2 antibody
    • Calmodulin 3 (phosphorylase kinase, delta) antibody
    • Calmodulin 3 antibody
    • CAM 2 antibody
    • CAM 3 antibody
    • CAM I antibody
    • CAM1 antibody
    • CAM2 antibody
    • CAM3 antibody
    • CAMB antibody
    • CAMC antibody
    • CAMI antibody
    • CAMII antibody
    • CAMIII antibody
    • CPVT4 antibody
    • DD132 antibody
    • FLJ99410 antibody
    • LP7057 protein antibody
    • PHKD antibody
    • PHKD2 antibody
    • PHKD3 antibody
    • phosphorylase kinase delta antibody
    • phosphorylase kinase, delta subunit antibody
    see all

Images

  • All lanes : Anti-Calmodulin 1/2/3 antibody (ab105498) at 1 µg/ml

    Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 16 kDa
    Observed band size: 16 kDa


    Exposure time: 20 minutes

References

This product has been referenced in:
  • Marciel MP  et al. Selenoprotein K deficiency inhibits melanoma by reducing calcium flux required for tumor growth and metastasis. Oncotarget 9:13407-13422 (2018). Read more (PubMed: 29568366) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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