Overview

  • Product nameAnti-Calmodulin 3 antibody [EPR5028]
    See all Calmodulin 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR5028] to Calmodulin 3
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues at the N-terminus of Human Calmodulin 3.

  • Positive control
    • HeLa, Ramos, HCT116 and NIH 3T3 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC or IHC-P.
  • Target

    • RelevanceCalmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
    • Cellular localizationSpindle. Note: Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
    • Database links
    • Alternative names
      • CALM antibody
      • CALM2 antibody
      • CALML2 antibody
      • Calmodulin 3 (phosphorylase kinase, delta) antibody
      • CAM antibody
      • CAM1 antibody
      • CAM2 antibody
      • CAM3 antibody
      • CAMB antibody
      • CAMC antibody
      • CAMIII antibody
      • PHKD antibody
      • PHKD3 antibody
      see all

    Anti-Calmodulin 3 antibody [EPR5028] images

    • Overlay histogram showing SHSY-5Y cells stained with ab124742 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124742, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-Calmodulin 3 antibody [EPR5028] (ab124742) at 1/50000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Ramos cell lysate
      Lane 3 : HCT116 cell lysate
      Lane 4 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 17 kDa
      Observed band size : 17 kDa

    References for Anti-Calmodulin 3 antibody [EPR5028] (ab124742)

    This product has been referenced in:
    • Simms BA  et al. A novel calmodulin site in the Cav1.2 N-terminus regulates calcium-dependent inactivation. Pflugers Arch 466:1793-803 (2014). WB ; Rat . Read more (PubMed: 24352630) »

    See 1 Publication for this product

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