Overview

  • Product nameAnti-Calmodulin antibody
    See all Calmodulin primary antibodies
  • Description
    Rabbit polyclonal to Calmodulin
  • SpecificityTargets C terminusof Calmodulin.
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (10-30 aa in length) conjugated to KLH and derived from the last C terminal 50 amino acids of Calmodulin (Human)

  • Positive control
    • Ramos cell lysate; Human breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab38590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • FunctionCalmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
  • Sequence similaritiesBelongs to the calmodulin family.
    Contains 4 EF-hand domains.
  • Post-translational
    modifications
    Ubiquitination results in a strongly decreased activity.
    Phosphorylation results in a decreased activity.
  • Cellular localizationCytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
  • Information by UniProt
  • Database links
  • FormThere are three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase.
  • Alternative names
    • CALM 1 antibody
    • CALM 2 antibody
    • CALM 3 antibody
    • CALM antibody
    • CALM_HUMAN antibody
    • CALM1 antibody
    • CALM2 antibody
    • Calm3 antibody
    • CALML2 antibody
    • calmodulin 1 (phosphorylase kinase, delta) antibody
    • Calmodulin 2 (phosphorylase kinase, delta) antibody
    • Calmodulin 3 (phosphorylase kinase, delta) antibody
    • Calmodulin antibody
    • CaM antibody
    • CAM I antibody
    • CAM1 antibody
    • CAM2 antibody
    • CAM3 antibody
    • CAMB antibody
    • CAMC antibody
    • CAMI antibody
    • CAMII antibody
    • CPVT4 antibody
    • DD132 antibody
    • FLJ99410 antibody
    • LP7057 protein antibody
    • PHKD antibody
    • PHKD2 antibody
    • PHKD3 antibody
    • phosphorylase kinase delta antibody
    • phosphorylase kinase, delta subunit antibody
    see all

Anti-Calmodulin antibody images

  • Anti-Calmodulin antibody (ab38590) + Ramos cell lysate

    Predicted band size : 17 kDa
    Observed band size : 17 kDa
    Additional bands at : 25 kDa,30 kDa. We are unsure as to the identity of these extra bands.
  • Formalin-fixed paraffin-embedded human cancer tissue (breast carcinoma) with ab38590 anti-calmodulin, peroxidase-conjugated to the secondary antibody, followed by AEC staining.

References for Anti-Calmodulin antibody (ab38590)

This product has been referenced in:
  • Hoedt E  et al. SILAC-based proteomic profiling of the human MDA-MB-231 metastatic breast cancer cell line in response to the two antitumoral lactoferrin isoforms: the secreted lactoferrin and the intracellular delta-lactoferrin. PLoS One 9:e104563 (2014). WB ; Human . Read more (PubMed: 25116916) »
  • Cohen AA  et al. Dynamic proteomics of individual cancer cells in response to a drug. Science 322:1511-6 (2008). WB ; Human . Read more (PubMed: 19023046) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (sarcoma)
Specification sarcoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH6, 20 min, 100C
Permeabilization No
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Submitted Dec 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"