Overview

  • Product name
    Anti-Calmodulin antibody [J4D8]
    See all Calmodulin primary antibodies
  • Description
    Mouse monoclonal [J4D8] to Calmodulin
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human Calmodulin (1-149aa) purified from E. coli

  • Positive control
    • NIH 3T3 cell lysate
  • General notes
    Myeloma cell line: mouse FO

Properties

Applications

Our Abpromise guarantee covers the use of ab75207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
  • Sequence similarities
    Belongs to the calmodulin family.
    Contains 4 EF-hand domains.
  • Post-translational
    modifications
    Ubiquitination results in a strongly decreased activity.
    Phosphorylation results in a decreased activity.
  • Cellular localization
    Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
  • Information by UniProt
  • Database links
  • Form
    There are three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase.
  • Alternative names
    • CALM 1 antibody
    • CALM 2 antibody
    • CALM 3 antibody
    • CALM antibody
    • CALM_HUMAN antibody
    • CALM1 antibody
    • CALM2 antibody
    • Calm3 antibody
    • CALML2 antibody
    • calmodulin 1 (phosphorylase kinase, delta) antibody
    • Calmodulin 2 (phosphorylase kinase, delta) antibody
    • Calmodulin 3 (phosphorylase kinase, delta) antibody
    • Calmodulin antibody
    • CaM antibody
    • CAM I antibody
    • CAM1 antibody
    • CAM2 antibody
    • CAM3 antibody
    • CAMB antibody
    • CAMC antibody
    • CAMI antibody
    • CAMII antibody
    • CPVT4 antibody
    • DD132 antibody
    • FLJ99410 antibody
    • LP7057 protein antibody
    • PHKD antibody
    • PHKD2 antibody
    • PHKD3 antibody
    • phosphorylase kinase delta antibody
    • phosphorylase kinase, delta subunit antibody
    see all

Images

  • Anti-Calmodulin antibody [J4D8] (ab75207) at 1/500 dilution + NIH3T3 cell lysates at 50 µg

    Secondary
    goat anti-mouse HRP
    Developed using the ECL technique

    Predicted band size : 17 kDa
    Observed band size : 17 kDa
  • Ab75207 staining Calmodulin in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Alexa Fluor® 488 was used as the secondary antibody. DAPI stain was used (blue).

References

This product has been referenced in:
  • Dionisio N  et al. Functional role of the calmodulin- and inositol 1,4,5-trisphosphate receptor-binding (CIRB) site of TRPC6 in human platelet activation. Cell Signal 23:1850-6 (2011). WB, IP ; Human . Read more (PubMed: 21757000) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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