The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
Use at an assay dependent dilution.
FunctionCalmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
Sequence similaritiesBelongs to the calmodulin family. Contains 4 EF-hand domains.
Post-translational modificationsUbiquitination results in a strongly decreased activity. Phosphorylation results in a decreased activity.
Cellular localizationCytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
Western blot - Calmodulin antibody [J4D8] (ab75207)
Anti-Calmodulin antibody [J4D8] (ab75207) at 1/500 dilution + NIH3T3 cell lysates at 50 µg
Secondary goat anti-mouse HRP Developed using the ECL technique
Predicted band size : 17 kDa Observed band size : 17 kDa
References for Anti-Calmodulin antibody [J4D8] (ab75207)
This product has been referenced in:
Dionisio N et al. Functional role of the calmodulin- and inositol 1,4,5-trisphosphate receptor-binding (CIRB) site of TRPC6 in human platelet activation. Cell Signal23:1850-6 (2011).
Read more (PubMed: 21757000) »