Overview

  • Product nameAnti-Calmodulin (phospho T79 + S81) antibody
    See all Calmodulin primary antibodies
  • Description
    Rabbit polyclonal to Calmodulin (phospho T79 + S81)
  • SpecificityDetects endogenous levels of Calmodulin only when phosphorylated at threonine 79 and serine 81.
  • Tested applicationsSuitable for: ICC/IF, ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Calmodulin. The antiserum was produced against synthesized phosphopeptide derived from human Calmodulin around the phosphorylation site of threonine 79 and serine 81 (K-D-T(p)-D-S(p)-E-E)
    Database link: P62158

  • Positive control
    • IHC-P: human brain tissue. WB: extracts from Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab61001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 40 kDa.
IHC-P 1/50 - 1/100.

Target

  • FunctionCalmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
  • Sequence similaritiesBelongs to the calmodulin family.
    Contains 4 EF-hand domains.
  • Post-translational
    modifications
    Ubiquitination results in a strongly decreased activity.
    Phosphorylation results in a decreased activity.
  • Cellular localizationCytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
  • Information by UniProt
  • Database links
  • FormThere are three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase.
  • Alternative names
    • CALM 1 antibody
    • CALM 2 antibody
    • CALM 3 antibody
    • CALM antibody
    • CALM_HUMAN antibody
    • CALM1 antibody
    • CALM2 antibody
    • Calm3 antibody
    • CALML2 antibody
    • calmodulin 1 (phosphorylase kinase, delta) antibody
    • Calmodulin 2 (phosphorylase kinase, delta) antibody
    • Calmodulin 3 (phosphorylase kinase, delta) antibody
    • Calmodulin antibody
    • CaM antibody
    • CAM I antibody
    • CAM1 antibody
    • CAM2 antibody
    • CAM3 antibody
    • CAMB antibody
    • CAMC antibody
    • CAMI antibody
    • CAMII antibody
    • CPVT4 antibody
    • DD132 antibody
    • FLJ99410 antibody
    • LP7057 protein antibody
    • PHKD antibody
    • PHKD2 antibody
    • PHKD3 antibody
    • phosphorylase kinase delta antibody
    • phosphorylase kinase, delta subunit antibody
    see all

Anti-Calmodulin (phospho T79 + S81) antibody images

  • All lanes : Anti-Calmodulin (phospho T79 + S81) antibody (ab61001) at 1/500 dilution

    Lane 1 : HeLa cells treated with Hu (1mg/ml)
    Lane 2 : Raw264.7 cells treated with TNFa (1mg/ml)

    Lysates/proteins at 15 µl per lane.
  • ab61001, at a 1/50 dilution, staining bi-phosphorylated Calmodulin on human brain tissue sections, in the presence (right) or absence (left) of the immunizing phosphopeptide.
  • Immunofluorescence analysis of HepG2 cells labeling Calmodulin (Phospho-Thr79+Ser81) with ab61001 at 1/100 dilution. The panel on the left is without the blocking peptide, while the panel on the right includes the blocking peptide.

References for Anti-Calmodulin (phospho T79 + S81) antibody (ab61001)

This product has been referenced in:
  • Pachuau J  et al. Protein kinase CK2 contributes to diminished small conductance Ca2+-activated K+ channel activity of hypothalamic pre-sympathetic neurons in hypertension. J Neurochem 130:657-67 (2014). Read more (PubMed: 24806793) »
  • Seo HS  et al. Phytoestrogens induce apoptosis via extrinsic pathway, inhibiting nuclear factor-kappaB signaling in HER2-overexpressing breast cancer cells. Anticancer Res 31:3301-13 (2011). Read more (PubMed: 21965740) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab61001.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"