• Product nameAnti-Calmodulin (phospho T79 + S81) antibody
    See all Calmodulin primary antibodies
  • Description
    Rabbit polyclonal to Calmodulin (phospho T79 + S81)
  • SpecificityDetects endogenous levels of Calmodulin only when phosphorylated at threonine 79 and serine 81.
  • Tested applicationsSuitable for: ICC/IF, ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Calmodulin. The antiserum was produced against synthesized phosphopeptide derived from human Calmodulin around the phosphorylation site of threonine 79 and serine 81 (K-D-T(p)-D-S(p)-E-E)
    Database link: P62158

  • Positive control
    • IHC-P: human brain tissue. WB: extracts from Jurkat cells.



Our Abpromise guarantee covers the use of ab61001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 40 kDa.
IHC-P 1/50 - 1/100.


  • FunctionCalmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
  • Sequence similaritiesBelongs to the calmodulin family.
    Contains 4 EF-hand domains.
  • Post-translational
    Ubiquitination results in a strongly decreased activity.
    Phosphorylation results in a decreased activity.
  • Cellular localizationCytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
  • Information by UniProt
  • Database links
  • FormThere are three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase.
  • Alternative names
    • CALM 1 antibody
    • CALM 2 antibody
    • CALM 3 antibody
    • CALM antibody
    • CALM_HUMAN antibody
    • CALM1 antibody
    • CALM2 antibody
    • Calm3 antibody
    • CALML2 antibody
    • calmodulin 1 (phosphorylase kinase, delta) antibody
    • Calmodulin 2 (phosphorylase kinase, delta) antibody
    • Calmodulin 3 (phosphorylase kinase, delta) antibody
    • Calmodulin antibody
    • CaM antibody
    • CAM I antibody
    • CAM1 antibody
    • CAM2 antibody
    • CAM3 antibody
    • CAMB antibody
    • CAMC antibody
    • CAMI antibody
    • CAMII antibody
    • CPVT4 antibody
    • DD132 antibody
    • FLJ99410 antibody
    • LP7057 protein antibody
    • PHKD antibody
    • PHKD2 antibody
    • PHKD3 antibody
    • phosphorylase kinase delta antibody
    • phosphorylase kinase, delta subunit antibody
    see all

Anti-Calmodulin (phospho T79 + S81) antibody images

  • All lanes : Anti-Calmodulin (phospho T79 + S81) antibody (ab61001) at 1/500 dilution

    Lane 1 : HeLa cells treated with Hu (1mg/ml)
    Lane 2 : Raw264.7 cells treated with TNFa (1mg/ml)

    Lysates/proteins at 15 µl per lane.
  • ab61001, at a 1/50 dilution, staining bi-phosphorylated Calmodulin on human brain tissue sections, in the presence (right) or absence (left) of the immunizing phosphopeptide.
  • Immunofluorescence analysis of HepG2 cells labeling Calmodulin (Phospho-Thr79+Ser81) with ab61001 at 1/100 dilution. The panel on the left is without the blocking peptide, while the panel on the right includes the blocking peptide.

References for Anti-Calmodulin (phospho T79 + S81) antibody (ab61001)

This product has been referenced in:
  • Pachuau J  et al. Protein kinase CK2 contributes to diminished small conductance Ca2+-activated K+ channel activity of hypothalamic pre-sympathetic neurons in hypertension. J Neurochem 130:657-67 (2014). Read more (PubMed: 24806793) »
  • Seo HS  et al. Phytoestrogens induce apoptosis via extrinsic pathway, inhibiting nuclear factor-kappaB signaling in HER2-overexpressing breast cancer cells. Anticancer Res 31:3301-13 (2011). Read more (PubMed: 21965740) »

See all 2 Publications for this product

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