Overview

  • Product nameAnti-Calnexin antibody
    See all Calnexin primary antibodies
  • Description
    Rabbit polyclonal to Calnexin
  • SpecificityRecognizes ER membrane, mitochondria and cis-Golgi
  • Tested applicationsSuitable for: WB, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human, Pig, Chinese Hamster
  • Immunogen

    His-Tag E. coli expressed N-terminal ER luminal domain of canine calnexin

Properties

Applications

Our Abpromise guarantee covers the use of ab10286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Predicted molecular weight: 67 kDa. 1/2000 - 1/5000. Predicted molecular weight: 67 kDa; however it does run on SDS-PAGE as an ~90kDa protein, this is mainly due to a number of negative charges at the C-terminus of the protein which effects the SDS binding to the molecules and consequently its SDS-PAGE mobility.
ICC 1/100.
ICC/IF Use at an assay dependent concentration.

Target

Anti-Calnexin antibody images



  • Predicted band size : 67 kDa


    Western blot probed with ab10286 at 1/1000. Lane 1 – calnexin deficient human NKR T-lymphoblastoid leukemia cell lines. Lane 2 - human CEM T-lymphoblastoid leukemia cell lines.
  • Anti-Calnexin antibody (ab10286) at 1/1000 dilution + MCF7 cells

    Secondary
    Goat anti rabbit at 1/10000 dilution

    Predicted band size : 67 kDa
  • All lanes : Anti-Calnexin antibody (ab10286) at 1/4000 dilution

    Lane 1 : HeLa (Total cell lysate)
    Lane 2 : HeLa (Membrane fraction)
    Lane 3 : HeLa (Cytoplasmic fraction)
    Lane 4 : MDA-MB231 (Total cell lysate)
    Lane 5 : MDA-MB231 (Membrane fraction)
    Lane 6 : MDA-MB231 (Cytoplasmic fraction)

    Secondary
    HRP conjugated donkey anti-rabbit
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 67 kDa
    Observed band size : 67 kDa


    Exposure time : 5 minutes

    This image is courtesy of an Abreview submitted by Dr Neil Taylor

    See Abreview

  • Paraformaldehyde fixed HeLa cells stained with ab10286. For further details please refer to the customer Abreview.

  • ab10286 staining Calnexin in Pig retinal pigment epithelial cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/500 in 0.1% TX100; 1% goat serum; 1XPBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat polyclonal to rabbit IgG, dilution 1/500, was used as secondary antibody.

    See Abreview

References for Anti-Calnexin antibody (ab10286)

This product has been referenced in:
  • Keller AC  et al. Varicella zoster virus infection of human fetal lung cells alters mitochondrial morphology. J Neurovirol 22:674-682 (2016). Read more (PubMed: 27245593) »
  • Rodin S  et al. Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment. Nat Commun 5:3195 (2014). Human . Read more (PubMed: 24463987) »

See all 20 Publications for this product

Product Wall

Application Western blot
Loading amount 11 µg
Gel Running Conditions Reduced Denaturing (12% Bis-Tris gel, MES buffer)
Sample Human Cell lysate - whole cell (HEK293T)
Specification HEK293T
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

Verified customer

Submitted Feb 23 2015

Abreviews
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Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Human Cell lysate - whole cell (Human endothelial cell line)
Specification Human endothelial cell line
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Jun 17 2013

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human Endothelial cells)
Specification Human Endothelial cells
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100 0.1% in blocking buffer
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Apr 10 2013

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Application Western blot
Sample Chinese Hamster Cell lysate - other (CHO cells)
Loading amount 15 µg
Specification CHO cells
Gel Running Conditions Reduced Denaturing (7.5 % Tris-Gly)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Mr. Yannick Waumans

Verified customer

Submitted Jun 29 2011

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Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (macrophages)
Specification macrophages
Fixative Formaldehyde
Permeabilization Yes - 0.2% triton x-100
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Submitted Nov 23 2010

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Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 10 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (12.5%)
Blocking step Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Nov 19 2010

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Application Western blot
Sample Mouse Cell lysate - whole cell (macrophages)
Loading amount 10 µg
Specification macrophages
Gel Running Conditions Reduced Denaturing (12.5%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Nov 12 2010

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Application Western blot
Sample Dog Cell lysate - whole cell (MDCK 2 cells)
Loading amount 25 µg
Specification MDCK 2 cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Herr Dr. Vladimir Milenkovic

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Submitted Oct 14 2010

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Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Macrophages)
Loading amount 20 µg
Specification Mouse Macrophages
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Sep 14 2010

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Application Western blot
Sample Rat Cell lysate - whole cell (Rat dendritic cells)
Loading amount 15 µg
Specification Rat dendritic cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Sep 10 2010

1-10 of 22 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"