Overview

  • Product nameAnti-Calnexin antibody
    See all Calnexin primary antibodies
  • Description
    Rabbit polyclonal to Calnexin
  • SpecificityRecognizes ER membrane, mitochondria and cis-Golgi
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Monkey
  • Immunogen

    Synthetic peptide:

    TAPPSSPKVTYKAPVPTGE

    conjugated to KLH, corresponding to amino acids 50-68 of Human Calnexin

  • Positive control
    • Heat Shocked HeLa Cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab13505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB 1/2000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
IP 1/100.

Target

Anti-Calnexin antibody images

  • ab13505 staining Calnexin - ER membrane marker in mouse back skin tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. The primary antibody was used at 1/100 dilution and a Fluorophore conjugated goat anti rabbit secondary at 1/50 dilution. 

  • ab13505 staining Calnexin - ER membrane marker in mouse colitis colon tissue section by immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ was used for staining. A HRP-conjugated anti rabbit was used at 1/10 dilution as secondary.
     

  • Anti-Calnexin antibody (ab13505) at 1/1000 dilution + Mixture of tissue lysates prepared from Rat tissues.

    Predicted band size : 90 kDa
  • Anti-Calnexin antibody (ab13505) at 1/2000 dilution ((in TBS with 0.05% TW-20)) + HeLa cell lysate

    Secondary
    An HRP-conjugated goat anti-rabbit IgG
    Developed using the ECL technique

    Predicted band size : 90 kDa
  • ab13505 staining Calnexin in HeLa cells by ICC/IF (Immunocytochemistry/immunofluoresence). Cells were fixed with 4% PFA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A Fluoroscein-conjugated goat anti-rabbit IgG secondary antibody was used.

References for Anti-Calnexin antibody (ab13505)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Thank you for your email and patience, and I'm sorry to hear that you are experiencing difficulty with this antibody. Below I have included the WB protocol that the originator used when testing ab13505. Please note that this is a general protocol, and ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"