• Product nameAnti-Calnexin antibody
    See all Calnexin primary antibodies
  • Description
    Rabbit polyclonal to Calnexin
  • Tested applicationsSuitable for: Flow Cyt, ICC/IF, IHC-P, IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Dog, Human, Pig, Drosophila melanogaster, Monkey, Quail
  • Immunogen

    Synthetic peptide corresponding to Dog Calnexin (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • HeLa cell lysate.
  • General notes

    References regarding specificity

    Horner SM  et al. Mitochondrial-associated endoplasmic reticulum membranes (MAM) form innate immune synapses and are targeted by hepatitis C virus. Proc Natl Acad Sci U S A 108:14590-5 (2011). PubMed: 21844353

    Myhill N  et al. The subcellular distribution of calnexin is mediated by PACS-2. Mol Biol Cell 19:2777-88 (2008). PubMed: 18417615

    Yoshimura SI  et al. Direct targeting of cis-Golgi matrix proteins to the Golgi apparatus. J Cell Sci 114:4105-15 (2001). PubMed: 11739642



Our Abpromise guarantee covers the use of ab75801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100.
IHC-P 1/100.
IHC-Fr 1/100.
IP 1/100.
WB 1/2000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).


Anti-Calnexin antibody images

  • Ab75801 staining Calnexin in juvenile mouse backskin tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • Anti-Calnexin antibody (ab75801) at 1/2000 dilution ((in TBS with 0.05% TW-20)) + HeLa cell lysate

    An HRP-conjugated goat anti-rabbit IgG
    developed using the ECL technique

    Predicted band size : 90 kDa
  • ab75801 used in Immunoprecipitation of Calnexin from Human Hela cells. The antibody was used at 20µl per 1 ml of cell lysate.

    20µl of protein G beads were used in Immunoprecipitation.  

  • ab75801 staining Calnexin in HeLa cells by ICC/IF (Immunocytochemistry/immunofluoresence). Cells were fixed with 4% PFA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A Fluoroscein-conjugated goat anti-rabbit IgG secondary antibody was used.

References for Anti-Calnexin antibody (ab75801)

This product has been referenced in:
  • Ulengin I  et al. ER network formation and membrane fusion by atlastin1/SPG3A disease variants. Mol Biol Cell 26:1616-28 (2015). Read more (PubMed: 25761634) »
  • Payne F  et al. Mutations disrupting the Kennedy phosphatidylcholine pathway in humans with congenital lipodystrophy and fatty liver disease. Proc Natl Acad Sci U S A 111:8901-6 (2014). Read more (PubMed: 24889630) »

See all 7 Publications for this product

Product Wall

ab13504 has been unpublished from our catalogue; however, ab75801 (http://www.abcam.com/calnexin-er-membrane-marker-antibody-ab75801.html) is the same antibody, in unpurified format, and it is listed to have weak cross reactivity with Drosophila c...

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Thank you for your inquiry.

I heard back from the lab that there is no sequence overlap between the immunogen for ab75801 and saccharomyces cerevisiae.

I looked through all our anti-Calnexin antibodies and the only one w...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 21°C

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Submitted Jun 03 2011