Anti-Calnexin antibody (ab93355)

Overview

  • Product nameAnti-Calnexin antibody
    See all Calnexin primary antibodies
  • Description
    Goat polyclonal to Calnexin
  • SpecificityRecognizes ER membrane, mitochondria and cis-Golgi
  • Tested applicationsSuitable for: WB, Sandwich ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Hamster, Cow, Dog, Pig, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Calnexin aa 169-183 (N terminal) (Cysteine residue).
    Sequence:

    SKTPELNLDQFHDKT

  • Positive control
    • Mouse heart lysate, Human brain cerebellum lysate, NIH3T3 and HeLa cell lysates
  • General notes

    References regarding specificity

    Horner SM  et al. Mitochondrial-associated endoplasmic reticulum membranes (MAM) form innate immune synapses and are targeted by hepatitis C virus. Proc Natl Acad Sci U S A 108:14590-5 (2011). PubMed: 21844353

    Myhill N  et al. The subcellular distribution of calnexin is mediated by PACS-2. Mol Biol Cell 19:2777-88 (2008). PubMed: 18417615

    Yoshimura SI  et al. Direct targeting of cis-Golgi matrix proteins to the Golgi apparatus. J Cell Sci 114:4105-15 (2001). PubMed: 11739642

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 68 kDa).
Sandwich ELISA Use a concentration of 1 - 2 µg/ml. Used as a reporter to detect captured recombinant Calnexin
IHC-P Use a concentration of 3 - 6 µg/ml.

Target

Anti-Calnexin antibody images

  • Anti-Calnexin antibody (ab93355) at 0.3 µg/ml + Mouse heart lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 68 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • ab93355 at 3.8 µg/ml staining Calnexin in Human Kidney by Immunohistochemistry (Formalin/PFA-Fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed, antibody was revealed by AP-staining.

References for Anti-Calnexin antibody (ab93355)

ab93355 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application IHC - Wholemount
Sample Rat Tissue (Brain stem)
Specification Brain stem
Username

Abcam user community

Verified customer

Submitted Jul 20 2012

A follow up message I received this morning from our IT department suggests there is something odd with your review. They are looking at it currently but they suggest trying to use your Abcam order reference number associated with the purchase of ab933...

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I'm so sorry submitting this review is proving to be difficult. Typically this process is quite smooth. I have again requested our IT team look at this issue, specifically regarding the lot number. They should get back to me by tomorrow and when they d...

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I have spoken with our IT department and they tell me that for some reason ug/ml was not being allowed as a valid concentration. This issue has been fixed so please complete the Abreview and resubmit. Hopefully it will work as expected but please conta...

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I recevied your voice mail today. I am sorry I was not able to call you back today.
And I apologize for the trouble you are having with the Abreview system. I will have our IT department look into it and follow up with you as soon as I have some a...

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Thank you for your reply.
Our recommended permeabilization protocol can be found here: http://www.abcam.com/index.html?pageconfig=resource&rid=11462
Triton or NP-40: Use 0.1 to 0.2% in PBS for 10 min only.
As for a different anti-calnexin a...

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Thank you for your feedback.
It does appear that you are getting some signal, but that perhaps the antibody is labeling the cell membrane? You may want to try permeabilizing the cells with Triton X-100 as a separate step befor the antibody incubati...

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DISCOUNT CODE: ***
Expiration date: October 23, 2012
This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab93355 in rat samples and include this code in the “Additiona...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"