Anti-Calnexin (phospho S583) antibody (ab58503)
Key features and details
- Rabbit polyclonal to Calnexin (phospho S583)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Calnexin (phospho S583) antibody
See all Calnexin primary antibodies -
Description
Rabbit polyclonal to Calnexin (phospho S583) -
Host species
Rabbit -
Specificity
ab58503 detects endogenous levels of Calnexin - ER membrane marker only when phosphorylated at serine 583. Recognizes ER membrane, mitochondria and cis-Golgi -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human Calnexin aa 500-600 (phospho S583).
Database link: P27824 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab58503 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). |
Target
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Function
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. -
Sequence similarities
Belongs to the calreticulin family. -
Cellular localization
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 821 Human
- Entrez Gene: 29144 Rat
- Omim: 114217 Human
- SwissProt: P27824 Human
- SwissProt: P35565 Rat
- Unigene: 567968 Human
- Unigene: 1762 Rat
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Alternative names
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab58503 has been referenced in 2 publications.
- Dallavilla T et al. Model-Driven Understanding of Palmitoylation Dynamics: Regulated Acylation of the Endoplasmic Reticulum Chaperone Calnexin. PLoS Comput Biol 12:e1004774 (2016). PubMed: 26900856
- Zhao Z TGFß and Wnt in Cardiac Outflow Tract Defects in Offspring of Diabetic Pregnancies. Birth Defects Res B Dev Reprod Toxicol 101:364-70 (2014). Mouse . PubMed: 25231192