Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Overview
Product name
Anti-Calpain 1 antibody - Aminoterminal end domain I
See all Calpain 1 products (13) ...
Description
Rabbit polyclonal to Calpain 1 - Aminoterminal end domain I
Specificity
ab28257 recognizes the amino terminal end of the large subunit of Calpain 1. The antibody does not recognize amino-processed Calpain 1, and can be used to discriminate between latent and amino-processed forms. Also, it does not cross react with the other calpain family members (M Calpain, Calpain 94, ncl2, ncl3, etc.).
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide based on the amino terminal end of the large subunit of human calpain 1
Epitope
ab28257 reacts with an epitope located in the first 30 amino acids of the propeptide domain, (Domain I) of Calpain 1, aminoterminal from the activation cleavage site.
Positive control
Human condrosarcoma cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody has been peptide-affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000.Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).(1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. But note that when used against the reduced protein, ab28257 identifies bands at 80 kDa, 68 kDa, and a series of further cleaved forms. Dilution optimised using Chromogenic detection.)
ICC/IF: Use a concentration of 1 - 5 µg/ml.
Target
Function
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 4 EF-hand domains.
Cellular localization
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
Target information above from: UniProt accessionP07384
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Ca2 activated neutral protease antibody
- Calcium activated neutral proteinase 1 antibody
- Calcium activated neutral proteinase antibody
see all
Database links
- Entrez Gene: 823 Human
- Entrez Gene: 12333 Mouse
- Entrez Gene: 29153 Rat
- Omim: 114220 Human
- SwissProt: P04632 Human
- SwissProt: P07384 Human
- SwissProt: O35350 Mouse
- SwissProt: P97571 Rat
see all
Anti-Calpain 1 antibody - Aminoterminal end domain I images:
Immunocytochemistry/ Immunofluorescence-Calpain 1 antibody - Aminoterminal end domain I(ab28257)
ICC/IF image of ab28257 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28257, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Calpain 1 antibody - Aminoterminal end domain I (ab28257)
All lanes : Anti-Calpain 1 antibody - Aminoterminal end domain I (ab28257) at 1 µg/ml
Lane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 82 kDa
Observed band size : 82 kDa
Additional bands at : 27 kDa,42 kDa,65 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
Protocols
References for Anti-Calpain 1 antibody - Aminoterminal end domain I (ab28257)
This product has been referenced in:
- Panigrahi AKet al. Calpain-1 cleaves Rad21 to promote sister chromatid separation. Mol Cell Biol 31:4335-47 (2011).Read more (PubMed: 21876002) »
- Li Yet al. Calpain 1 and Calpastatin expression is developmentally regulated in rat brain. Exp Neurol 220:316-9 (2009). WB; Rat.Read more (PubMed: 19751724) »
See all 3 publications for this product
Publishing research using ab28257? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"