| | | | Immunocytochemistry/ Immunofluorescence-Calpain 1 antibody - Domain IV(ab39170)
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| Product Name | Calpain 1 antibody - Domain IV |
| | See all Calpain 1 antibodies (9)... |
| Product type | Primary antibodies |
| Description | Rabbit polyclonal to Calpain 1 - Domain IV |
| Immunogen | Synthetic peptide based on Domain IV of the large subunit of Human Calpain 1.(Peptide available as ab41300.) |
| Reacts with
(species key) | Hu, Ms, Rat |
| Specificity | This antibody binds to reduced and non reduced Calpain 1. It reacts with Domain IV of Calpain 1, and detects both latent and aminoprocessed (activated) Calpain 1. It but does not cross react with the other calpain family members (Calpain 2, calpain 94, ncl 2, ncl 3, etc.). We have a range of domain specific antibodies for this target. For a full list please see all Calpain 1 antibodies |
| Tested applications
(see key) | ICC/IF, Immunohistochemistry (PFA fixed), WB |
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| Application notes
(see key) | Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/1000 - 1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 82 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Positive control
(see definition) | Cell lysates from human chondrosarcoma. |
| Cellular localization | Cytoplasmic. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding. |
| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain |
| Relevance | Mu Calpain, also known as Calpain I, and m calpain, also known as Calpain II, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. The calpains have papain like activity, thus the -pain nomenclature. Both m calpain and mu calpain are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV.
The calpains, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach specific, and muscle specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. |
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| Database links | The links below go to external sites and will open in a new browser window |
| Raised in | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Immunogen affinity purified |
| Storage buffer | Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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| Form | Liquid |
| Concentration | 1.000 mg/ml |
| Storage instructions | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles. |
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Calpain 1 antibody - Domain IV is on this datasheet. But please do contact us if you would like any reassurance! |
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| See below for Calpain 1 antibody - Domain IV images, references, products related to ab39170 and other tools. |
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Calpain 1 antibody - Domain IV images: |
 Immunocytochemistry/ Immunofluorescence-Calpain 1 antibody - Domain IV(ab39170)
ICC/IF image of ab39170 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39170, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for Calpain 1 antibody - Domain IV (ab39170) |
|  This product has been used in:
Nuzzi PA et al. Asymmetric localization of calpain 2 during neutrophil chemotaxis. Mol Biol Cell 18:795-805 (2007). PubMed: 17192410
If you publish research using ab39170 please let us know so that we can cite the reference on this datasheet.
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| Search PubMed (MEDLINE) for references to Calpain 1 |
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