Anti-Calpain 1 antibody [EPR3323] (ab92356)

Overview

  • Product nameAnti-Calpain 1 antibody [EPR3323]
    See all Calpain 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3323] to Calpain 1
  • SpecificityDetects Calpain 1 small subunit
  • Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Calpain 1 (small subunit).

  • Positive control
    • T47-D, 293T, A431 and Human spleen lysates. Human kidney tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab92356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC
Flow Cyt
  • Application notesFlow Cyt: 1/100.
    ICC: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. Antigen retrieval is recommended. The use of an HRP/AP polymerized antibody is recommended as these have been shown to give superior staining.

    WB: 1/1000 - 1/2000. Predicted molecular weight: 28 kDa.

    Is unsuitable for IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCalcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
    • Tissue specificityUbiquitous.
    • Sequence similaritiesBelongs to the peptidase C2 family.
      Contains 1 calpain catalytic domain.
      Contains 4 EF-hand domains.
    • Cellular localizationCytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ca2 activated neutral protease antibody
      • Calcium activated neutral proteinase antibody
      • Calcium activated neutral proteinase small subunit antibody
      • Calcium dependent protease small subunit 1 antibody
      • Calcium dependent protease small subunit antibody
      • Calcium-activated neutral proteinase 1 antibody
      • Calpain 1 antibody
      • Calpain 1 large subunit antibody
      • Calpain 1, (mu/I) large subunit antibody
      • Calpain mu type antibody
      • Calpain mu-type antibody
      • Calpain regulatory subunit antibody
      • Calpain small subunit 1 antibody
      • Calpain, large polypeptide L1 antibody
      • Calpain-1 catalytic subunit antibody
      • Calpain-1 large subunit antibody
      • CAN1_HUMAN antibody
      • CANP 1 antibody
      • CANP antibody
      • CANP small subunit antibody
      • CANP1 antibody
      • CANPL 1 antibody
      • CANPL1 antibody
      • CAPN 1 antibody
      • CAPN1 antibody
      • Cell proliferation inducing protein 30 antibody
      • Cell proliferation-inducing gene 30 protein antibody
      • Micromolar Calpain antibody
      • Micromolar-calpain antibody
      • Mu Calpain antibody
      • muCANP antibody
      • muCL antibody
      see all

    Anti-Calpain 1 antibody [EPR3323] images

    • All lanes : Anti-Calpain 1 antibody [EPR3323] (ab92356) at 1/1000 dilution

      Lane 1 : T47-D cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size : 28 kDa
    • ab92356 at 1/100 dilution staining Calpain 1 in paraffin-embedded Human kidney

    References for Anti-Calpain 1 antibody [EPR3323] (ab92356)

    ab92356 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab92356.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"