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Product name Anti-Calpain 1 antibody [EPR3323] See all Calpain 1 primary antibodies
Rabbit monoclonal [EPR3323] to Calpain 1
Specificity Detects Calpain 1 small subunit
Tested applications Suitable for:
WB, IHC-P, ICC, Flow Cyt more details Unsuitable for:
Mouse, Rat, Human
A synthetic peptide corresponding to residues in Human Calpain 1 (small subunit).
T47-D, 293T, A431 and Human spleen lysates. Human kidney tissue.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity Tissue culture supernatant
Clone number EPR3323
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes Flow Cyt: 1/100.
ICC: 1/100 - 1/250.
IHC-P: 1/100 - 1/250. Antigen retrieval is recommended. The use of an HRP/AP polymerized antibody is recommended as these have been shown to give superior staining.
WB: 1/1000 - 1/2000. Predicted molecular weight: 28 kDa.
Is unsuitable for IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Function Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Tissue specificity Ubiquitous.
Sequence similarities Belongs to the peptidase C2 family. Contains 1 calpain catalytic domain. Contains 4 EF-hand domains.
Cellular localization Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
Information by UniProt
Ca2 activated neutral protease antibody
Calcium activated neutral proteinase antibody
Calcium activated neutral proteinase small subunit antibody
Anti-Calpain 1 antibody [EPR3323] images
Western blot - Calpain 1 antibody [EPR3323] (ab92356)
All lanes : Anti-Calpain 1 antibody [EPR3323] (ab92356) at 1/1000 dilution Lane 1 : T47-D cell lysate Lane 2 : 293T cell lysate Lane 3 : A431 cell lysate Lane 4 : Human spleen lysate Lysates/proteins at 10 µg per lane. Secondary HRP labelled goat anti-rabbit antibody at 1/2000 dilution Predicted band size : 28 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calpain 1 antibody [EPR3323] (ab92356)
ab92356 at 1/100 dilution staining Calpain 1 in paraffin-embedded Human kidney
References for Anti-Calpain 1 antibody [EPR3323] (ab92356)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"