For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Calpain 1 Inhibitor Screening Kit (ab155888) is based on the detection of cleavage of calpain substrate Ac-LLY-AFC. Ac-LLY-AFC possess very weak fluorescence; upon cleavage of the substrate by calpain 1, free AFC can be quantified fluorometrically (Ex/Em = 400/505 nm). In the presence of potent Calpain 1 inhibitor, the hydrolyzation of substrate will be impeded. The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput screening of Calpain 1 inhibitors. Inhibitor control (Calpain 1 inhibitor ) is included to compare the efficacy of test inhibitors.
Calpain 1 is a calcium-dependent, non-lysosomal cysteine protease with low calcium requirement. It is expressed ubiquitously and in the brain, mainly localized to the cell body and dendrites of neurons and to a lesser extent in axons and glial cells. Hyperactivation of calpains is implicated in a number of pathologies associated with altered calcium homeostasis such as Alzheimer’s disease as well as secondary degeneration resulting from acute cellular stress following cerebral (neuronal) ischemia, traumatic brain injury and spinal cord injury.
|Calpain 1 Assay Buffer||WM||1 x 25ml|
|Calpain 1 Enzyme||Green||1 x 0.2ml|
|Calpain 1 Inhibitor Control||Blue||1 x 0.5ml|
|Calpain 1 Substrate||Red||1 x 0.2ml|
Our Abpromise guarantee covers the use of ab155888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab155888 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"