Customer reviews and FAQs

Overview

  • Product name
    Anti-Calpain 10 antibody
    See all Calpain 10 primary antibodies
  • Description
    Rabbit polyclonal to Calpain 10
  • Specificity
    This antibody does not cross react with the other calpain family members (calpain 1, calpain 2, calpain 3, etc.) It recognizes latent and amino-processed Calpain 10. This antibody binds to the middle of the catalytic domain, aminoterminal from the catalytic histidine.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Rat) based on the Catalytic domain of the large subunit. (Peptide available as ab41360.)

Properties

Applications

Our Abpromise guarantee covers the use of ab28220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 78 66 kDa (predicted molecular weight: 75 kDa). A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Defects in CAPN10 are a cause of susceptibility to diabetes mellitus non-insulin-dependent type 1 (NIDDM1) [MIM:601283]. It is a multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • Sequence similarities
    Belongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Calcium activated neutral protease antibody
    • Calcium activated neutral proteinase 10 antibody
    • Calcium-activated neutral proteinase 10 antibody
    • calpain like protease CAPN10 antibody
    • Calpain-10 antibody
    • Calpain10 antibody
    • CAN10_HUMAN antibody
    • CANP 10 antibody
    • CANP10 antibody
    • CAPN 10 antibody
    • CAPN10 antibody
    • EC 3.4.22. antibody
    • KIAA1845 antibody
    • NIDDM1 antibody
    see all

Anti-Calpain 10 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Calpain 10 antibody - Catalytic domain (Domain II)(ab28220)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Calpain 10 antibody - Catalytic domain (Domain II)(ab28220)
    IHC image of ab28220 staining in human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28220, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Calpain 10 antibody (ab28220)

ab28220 has not yet been referenced specifically in any publications.

Publishing research using ab28220? Please let us know so that we can cite the reference in this datasheet.

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Western blot abreview for Anti-Calpain 10 antibody

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293 HEK (human embryonal kidney fibroblasts))
Loading amount
100 µg
Specification
293 HEK (human embryonal kidney fibroblasts)
Treatment
0.7 µM Insulin for 5 min
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 22°C
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Submitted Mar 05 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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