Calpain 10 antibody - Aminoterminal end domain I (ab28222)

Product Name 

Calpain 10 antibody - Aminoterminal end domain I

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calpain 10 - Aminoterminal end domain I

Immunogen 

Synthetic peptide (Human) based on domain-I of the large subunit.

(Peptide available as ab44842.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

The antibody binds to RP3Calpain-10, but does not cross react with the other calpain family members (calpain-1, calpain-2, calpain-3, etc.).

We have a range of domain specific antibodies for this target. For a full list please see all Calpain 10 antibodies

Epitope 

ab28222 binds to the aminoterminal end of domain-I.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 - 1/5000. Detects a band of approximately 78 kDa (predicted molecular weight: 75 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain

Relevance 

Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain.

Calpain 10 is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. It is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM) and the gene is located within the NIDDM1 region. Multiple alternative transcript variants encoding different isoforms have been described.

This antibody recognizes latent but not amino-processed Calpain-10.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C. Aliquot and store at -20°C or -80°C when reconstituted. Avoid repeated freeze / thaw cycles.

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