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Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Overview
Product name
Anti-Calpain 10 antibody - Aminoterminal end domain I
See all Calpain 10 products (6) ...
Description
Rabbit polyclonal to Calpain 10 - Aminoterminal end domain I
Specificity
The antibody binds to RP3Calpain-10, but does not cross react with the other calpain family members (calpain-1, calpain-2, calpain-3, etc.).
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (Human) based on domain-I of the large subunit.
(Peptide available as ab44842.)
Epitope
ab28222 binds to the aminoterminal end of domain-I.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Aliquot and store at -20°C or -80°C when reconstituted. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
This antibody recognizes latent but not amino-processed Calpain-10.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
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Immunocytochemistry/ Immunofluorescence-Calpain 10 antibody - Aminoterminal end domain I(ab28222)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab28222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
- 1 Image
ICC/IF
Application notes
ICC/IF: Use at a concentration of 1 - 5 µg/ml.
WB: 1/1000 - 1/5000. Detects a band of approximately 78 kDa (predicted molecular weight: 75 kDa). Dilution optimised using Chromogenic detection.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. Calpain 10 is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. Calpain 10 is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Calpain-10 may represent the third example of a protease contributing to the development of diabetes, the others being prohormone-processing carboxypeptidase E and prohormone convertase-1, both of which are associated with diabetes and obesity. Multiple alternative transcript variants have been described for this gene.
Cellular localization
Cytoplasmic and Plasma membrane
Alternative names
- Calcium activated neutral proteinase 10 antibody
- CANP 10 antibody
- Calcium activated neutral protease antibody
see all
Database links
- Entrez Gene: 11132 Human
- Entrez Gene: 23830 Mouse
- Entrez Gene: 63834 Rat
- Omim: 605286 Human
- SwissProt: Q9HC96 Human
- SwissProt: Q9ESK3 Mouse
- SwissProt: Q9ES66 Rat
- Unigene: 112218 Human
see all
Anti-Calpain 10 antibody - Aminoterminal end domain I images:
Immunocytochemistry/ Immunofluorescence-Calpain 10 antibody - Aminoterminal end domain I(ab28222)
ICC/IF image of ab28222 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28222, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References for Anti-Calpain 10 antibody - Aminoterminal end domain I (ab28222)
ab28222 has not yet been referenced specifically in any publications.
Publishing research using ab28222? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"