Calpain 10 antibody - Aminoterminal end domain T (ab38965)

Product Name 

Calpain 10 antibody - Aminoterminal end domain T

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calpain 10 - Aminoterminal end domain T

Immunogen 

Synthetic peptide based on domain-T of the large subunit of human Calpain 10.
(Peptide available as ab44844.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

The antibody does not cross react with the other calpain family members (calpain-1, calpain-2, calpain-3, etc.). The antibody recognizes latent and amino-processed Calpain 10.

We have a range of domain specific antibodies for this target. For a full list please see all Calpain 10 antibodies

Epitope 

ab38965 binds to the aminoterminal end of domain-T.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. When used against the reduced protein, Ab38965 identifies bands at 78 Kd, 66 Kd, and a series of further cleaved forms. Many cell types produce RP4Calpain-10; since it is a cellular protein, cell lysate works well for Western blots. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain

Relevance 

Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain.

Calpain 10 is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. It is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM) and the gene is located within the NIDDM1 region. Multiple alternative transcript variants encoding different isoforms have been described.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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