Calpain 10 antibody - Domain III, large subunit (ab28226)

Product Name 

Calpain 10 antibody - Domain III, large subunit

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calpain 10 - Domain III, large subunit

Immunogen 

Synthetic peptide based on the aminoterminal end of domain-III in the large subunit, using the human sequence.

(Peptide available as ab41361.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

The antibody binds to Calpain-10, but does not cross react with the other calpain family members (Calpain-1, Calpain-2, Calpain-3, etc.).
The antibody binds to the aminoterminal end of domain-III and recognizes latent and amino-processed Calpain-10.

We have a range of domain specific antibodies for this target. For a full list please see all Calpain 10 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. Predicted molecular weight: 74.9 kDa. Post-translational modifications such as phosphorylation can produce isoforms with larger apparent MW on SDS PAGE gels. When used against the reduced protein, ab28226 identifies bands at 78 kD, 66 kD, and a series of further cleaved forms. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain

Relevance 

Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain.

Calpain 10 is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. It is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM) and the gene is located within the NIDDM1 region. Multiple alternative transcript variants encoding different isoforms have been described.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

References for Calpain 10 antibody - Domain III, large subunit (ab28226)

  This product has been used in:

Covington MD et al. Calpain 10 is required for cell viability and is decreased in the aging kidney. Am J Physiol Renal Physiol 296:F478-86 (2009). PubMed: 19144693

If you publish research using ab28226 please let us know so that we can cite the reference on this datasheet.

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