Calpain 11 antibody - Domain II (catalytic domain), large subunit (ab28227)

Product Name 

Calpain 11 antibody - Domain II (catalytic domain), large subunit

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calpain 11 - Domain II (catalytic domain), large subunit

Immunogen 

Synthetic peptide based on domain-III in the large subunit, using the human sequence.

(Peptide available as ab46846.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

The antibody binds to Calpain 11, but does not cross react with the other calpain family members (calpain 1, Calpain 2, ncl 2, ncl 3, etc.)

The antibody recognizes latent and the aminotruncated (active) Calpain and will also bind to the non-reduced protein.

We have a range of domain specific antibodies for this target. For a full list please see all Calpain 11 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species Predicted molecular weight: 81 kDa. The latent large subunit migrates at about 81 kD on reduced SDS PAGE gels, and truncation at the amino terminal end yields an approximately 58 Kd form. A cascade of smaller forms can be seen with further activation. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Intracellular

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain

Relevance 

Calpain 11 is an intracellular, calcium-dependent cysteine protease. Calpain 11 was first described in the testis, and more weakly in mammary gland and thymus, and maps to chromosome 6. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV, making calpain 11 most similar to the "classical" calpains 1 & 2. Sequence alignments place calpain 11 closest to chicken calpain u/m, and it may be that calpain 11 is the human orthologue of this protein.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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