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| Product Name | Calpain 11 antibody - Domain III, large subunit |
| | See all Calpain 11 antibodies (5)... |
| Product type | Primary antibodies |
| Description | Rabbit polyclonal to Calpain 11 - Domain III, large subunit |
| Immunogen | Synthetic peptide based on the carboxy terminal end of domain-III in the large subunit, using the human sequence. |
| Reacts with
(species key) | Hu, Ms, Rat |
| Specificity | The antibody binds to Calpain 11, but does not cross react with the other calpain family members (calpain 1, Calpain 2, ncl 2, ncl 3, etc.).
This antibody recognizes latent and the aminotruncated (active) Calpain 11. A cascade of smaller forms can be seen with further activation. ab28221 will also bind to the non-reduced protein. We have a range of domain specific antibodies for this target. For a full list please see all Calpain 11 antibodies |
| Tested applications
(see key) | WB |
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| Abreviews | |
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| Application notes
(see key) | Recommended dilutions
WB: 1/1000 for colorimetric substrates such as BCIP/NBT and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species Predicted molecular weight: 81 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Cellular localization | Intracellular |
| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain |
| Relevance | Calpain 11 is an intracellular, calcium-dependent cysteine protease. Calpain 11 was first described in the testis, and more weakly in mammary gland and thymus, and maps to chromosome 6. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV, making calpain 11 most similar to the "classical" calpains 1 & 2. Sequence alignments place calpain 11 closest to chicken calpain u/m, and it may be that calpain 11 is the human orthologue of this protein. |
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| Database links | The links below go to external sites and will open in a new browser window |
| Raised in | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Immunogen affinity purified |
| Storage buffer | Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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| Form | Liquid |
| Concentration | 1.000 mg/ml |
| Storage instructions | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Calpain 11 antibody - Domain III, large subunit is on this datasheet. But please do contact us if you would like any reassurance! |
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| Search PubMed (MEDLINE) for references to Calpain 11 |
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