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Anti-Calpain 11 antibody - Domain III, large subunit (ab28221)

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Overview

Product name

Anti-Calpain 11 antibody - Domain III, large subunit
See all Calpain 11 products (5) ...

Description

Rabbit polyclonal to Calpain 11 - Domain III, large subunit

Specificity

The antibody binds to Calpain 11, but does not cross react with the other calpain family members (calpain 1, Calpain 2, ncl 2, ncl 3, etc.). This antibody recognizes latent and the aminotruncated (active) Calpain 11. A cascade of smaller forms can be seen with further activation. ab28221 will also bind to the non-reduced protein.

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide based on the carboxy terminal end of domain-III in the large subunit, using the human sequence.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain

Applications

Show applications key

Our Abpromise guarantee covers the use of ab28221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     

Application notes

WB: 1/1000 for colorimetric substrates such as BCIP/NBT and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species Predicted molecular weight: 81 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Calpain 11 is an intracellular, calcium-dependent cysteine protease. Calpain 11 was first described in the testis, and more weakly in mammary gland and thymus, and maps to chromosome 6. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV, making calpain 11 most similar to the "classical" calpains 1 & 2. Sequence alignments place calpain 11 closest to chicken calpain u/m, and it may be that calpain 11 is the human orthologue of this protein.

Cellular localization

Intracellular

Alternative names

  • Calcium activated neutral proteinase 11 antibody
  • CAPN 11 antibody
  • CAPN11 antibody

Database links

see all

Protocols

  • Recommended WB protocol

    • References for Anti-Calpain 11 antibody - Domain III, large subunit (ab28221)

    ab28221 has not yet been referenced specifically in any publications.

    Publishing research using ab28221? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"