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Anti-Calpain 12 antibody - Aminoterminal end domain IV, large subunit (ab28232)

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Overview

Product name

Anti-Calpain 12 antibody - Aminoterminal end domain IV, large subunit
See all Calpain 12 products (4) ...

Description

Rabbit polyclonal to Calpain 12 - Aminoterminal end domain IV, large subunit

Specificity

This antibody binds to Calpain-12, but does not cross react with the other calpain family members (calpain-1, Calpain-2, ncl-2, ncl-3, etc.).

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide based on Mouse Calpain 12 domain-IV in the large subunit. (Peptide available as ab41368.)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins

Applications

Show applications key

Our Abpromise guarantee covers the use of ab28232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     

Application notes

WB: 1:1000 when using colorimetric substrates such as BCIP/NBT and 1:5000 for chemiluminescent substrates. Predicted molecular weight: 81 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Calpains are a family of cytosolic calcium activated cysteine proteases involved in a variety of cellular processes including apoptosis, cell division, modulation of integrin and cytoskeletal interactions, and synaptic plasticity. Calpain 12 was first described in the mouse, most strongly in the skin, and maps to mouse chromosome 7. Isoforms differ in the carboxyterminal ends, ending with aberrant domain III and lacking domain IV. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV, making Calpain 12 most similar to calpains 1 and 2.

Cellular localization

Cytoplasmic and Plasma membrane

Alternative names

  • Calpain12 antibody
  • CAPN 12 antibody
  • CAPN12 antibody
  • EC 3.4.22. antibody
  • MGC20576 antibody
see all

Database links

Protocols

  • Recommended WB protocol

    • References for Anti-Calpain 12 antibody - Aminoterminal end domain IV, large subunit (ab28232)

    ab28232 has not yet been referenced specifically in any publications.

    Publishing research using ab28232? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"