Calpain 12 antibody - Domain I, large subunit (ab28229)

Product Name 

Calpain 12 antibody - Domain I, large subunit

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calpain 12 - Domain I, large subunit

Immunogen 

Synthetic peptide (Mouse) based on the amino terminal end of domain-I in the large subunit.(Peptide available as ab41366.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

The antibody binds to Calpain-12, but does not cross react with the other calpain family members (calpain-1, Calpain-2, ncl-2, ncl-3, etc.).

We have a range of domain specific antibodies for this target. For a full list please see all Calpain 12 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 - 1/5000. Predicted molecular weight: 81 kDa. The latent large subunit migrates at about 80 kD on reduced SDS PAGE gels, and truncation at the amino terminal end yields an approximately 58 kDa form. A cascade of smaller cleaved forms can be seen with further activation. The 720 amino acid sequence predicts a protein of 80.588 kD, and the truncated 502, 462 and 449 amino acid sequences predict proteins of 55.874, 51.492 and 50.397 kD respectively.

A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic and Plasma membrane

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins

Relevance 

Calpains are a family of cytosolic calcium activated cysteine proteases involved in a variety of cellular processes including apoptosis, cell division, modulation of integrin and cytoskeletal interactions, and synaptic plasticity.

Calpain 12 was first described in the mouse, most strongly in the skin, and maps to mouse chromosome 7. Isoforms differ in the carboxyterminal ends, ending with aberrant domain III and lacking domain IV. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV, making Calpain 12 most similar to calpains 1 and 2.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C. Aliquot and store at -20°C or -80°C when reconstituted. Avoid repeated freeze / thaw cycles.

Notes 

Diluted antibody can be reused several times if not contaminated; store at 4°C with 0.05% Sodium Azide as preservative.

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