Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Overview
Product name
Anti-Calpain 12 antibody - Domain I, large subunit
See all Calpain 12 products (4) ...
Description
Rabbit polyclonal to Calpain 12 - Domain I, large subunit
Specificity
The antibody binds to Calpain-12, but does not cross react with the other calpain family members (calpain-1, Calpain-2, ncl-2, ncl-3, etc.).
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (Mouse) based on the amino terminal end of domain-I in the large subunit. (Peptide available as ab41366.)
General notes
Diluted antibody can be reused several times if not contaminated; store at 4°C with 0.05% Sodium Azide as preservative.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Aliquot and store at -20°C or -80°C when reconstituted. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/1000 - 1/5000. Predicted molecular weight: 81 kDa. The latent large subunit migrates at about 80 kD on reduced SDS PAGE gels, and truncation at the amino terminal end yields an approximately 58 kDa form. A cascade of smaller cleaved forms can be seen with further activation. The 720 amino acid sequence predicts a protein of 80.588 kD, and the truncated 502, 462 and 449 amino acid sequences predict proteins of 55.874, 51.492 and 50.397 kD respectively.
A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Calpains are a family of cytosolic calcium activated cysteine proteases involved in a variety of cellular processes including apoptosis, cell division, modulation of integrin and cytoskeletal interactions, and synaptic plasticity. Calpain 12 was first described in the mouse, most strongly in the skin, and maps to mouse chromosome 7. Isoforms differ in the carboxyterminal ends, ending with aberrant domain III and lacking domain IV. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV, making Calpain 12 most similar to calpains 1 and 2.
Cellular localization
Cytoplasmic and Plasma membrane
Alternative names
- Calpain12 antibody
- CAPN 12 antibody
- CAPN12 antibody
see all
Database links
- Entrez Gene: 147968 Human
- Entrez Gene: 60594 Mouse
- Omim: 608839 Human
- SwissProt: Q6ZSI9 Human
- SwissProt: Q9ER56 Mouse
- Unigene: 528327 Human
- Unigene: 589595 Human
- Unigene: 81144 Mouse
Protocols
References for Anti-Calpain 12 antibody - Domain I, large subunit (ab28229)
ab28229 has not yet been referenced specifically in any publications.
Publishing research using ab28229? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"