Calpain 12 antibody - Domain III, large subunit (ab38947)

Product Name 

Calpain 12 antibody - Domain III, large subunit

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calpain 12 - Domain III, large subunit

Immunogen 

Synthetic peptide based on the calcium binding region of domain-III in the large subunit of Mouse Calpain 12.

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab38947 binds to Calpain 12, but does not cross react with the other calpain family members (calpain 1, Calpain 2, ncl 2, ncl 3, etc.)
ab38947 recognizes latent and the aminotruncated (active) Calpain 12, and recognizes all but the shortest (449 AA) form of calpain 12.

We have a range of domain specific antibodies for this target. For a full list please see all Calpain 12 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. When used against the reduced protein this antibody identifies bands at 80 Kd, and a series of further cleaved forms. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic and Plasma membrane

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins

Relevance 

Calpains are a family of cytosolic calcium activated cysteine proteases involved in a variety of cellular processes including apoptosis, cell division, modulation of integrin and cytoskeletal interactions, and synaptic plasticity.

Calpain 12 was first described in the mouse, most strongly in the skin, and maps to mouse chromosome 7. Isoforms differ in the carboxyterminal ends, ending with aberrant domain III and lacking domain IV. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV, making Calpain 12 most similar to calpains 1 and 2.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

ab38947 has been peptide-affinity purified.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Store diluted antibody at 4°C with 0.05% Sodium Azide as preservative.

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