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Anti-Calpain 13 antibody - Domain I, large subunit (ab28234)

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Overview

Product name

Anti-Calpain 13 antibody - Domain I, large subunit
See all Calpain 13 products (2) ...

Description

Rabbit polyclonal to Calpain 13 - Domain I, large subunit

Specificity

This antibody binds to Calpain 13, but does not cross react with the other calpain family members Calpain 1, Calpain 2, ncl 2, ncl 3, etc. It recognizes latent but not the aminotruncated (active) Calpain-13. All three forms of calpain-13 are recognized.

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide (Human) based on the amino terminal end of domain-I in the large subunit. (Peptide available as ab41369.)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain

Applications

Show applications key

Our Abpromise guarantee covers the use of ab28234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     

Application notes

WB: 1:1000 when using colorimetric substrates such as BCIP/NBT and 1:5000 for chemiluminescent substrates. Predicted molecular weight: 77 kDa. When using reducing conditions a band at about 80kDa is observed and a series of cleaved forms may also be seen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Calpain 13 is an intracellular, calcium-dependent cysteine protease. Calpain 13 was first described in human EST databases, and maps to mouse chromosome 2. The different isoforms differ in the carboxyterminal ends, ending with aberrant domain-III and lacking domain IV. Calpain 13 looks similar to the other calpain members in domains I and II, but domain-III lacks the polyglutamic acid stretch that aids in calcium binding, and domain-IV is very different. Sequence alignments place Calpain 13 closest to calpain 14, also found on chromosome 2.

Cellular localization

Intracellular

Alternative names

  • CAPN 13 antibody
  • CAPN13 antibody
  • FLJ23523 antibody
  • Gm943 antibody
  • Hypothetical protein CAPN13 antibody
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Database links

see all

Protocols

  • Recommended WB protocol

    • References for Anti-Calpain 13 antibody - Domain I, large subunit (ab28234)

    ab28234 has not yet been referenced specifically in any publications.

    Publishing research using ab28234? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"