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| Product Name | Calpain 13 antibody - Domain I, large subunit |
| | See all Calpain 13 antibodies (2)... |
| Product type | Primary antibodies |
| Description | Rabbit polyclonal to Calpain 13 - Domain I, large subunit |
| Immunogen | Synthetic peptide (Human) based on the amino terminal end of domain-I in the large subunit.(Peptide available as ab41369.) |
| Reacts with
(species key) | Hu, Ms, Rat |
| Specificity | This antibody binds to Calpain 13, but does not cross react with the other calpain family members Calpain 1, Calpain 2, ncl 2, ncl 3, etc. It recognizes latent but not the aminotruncated (active) Calpain-13. All three forms of calpain-13 are recognized. We have a range of domain specific antibodies for this target. For a full list please see all Calpain 13 antibodies |
| Tested applications
(see key) | WB |
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| Abreviews | |
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| Application notes
(see key) | Recommended dilutions
WB: 1:1000 when using colorimetric substrates such as BCIP/NBT and 1:5000 for chemiluminescent substrates. Predicted molecular weight: 77 kDa. When using reducing conditions a band at about 80kDa is observed and a series of cleaved forms may also be seen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Cellular localization | Intracellular |
| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain |
| Relevance | Calpain 13 is an intracellular, calcium-dependent cysteine protease. Calpain 13 was first described in human EST databases, and maps to mouse chromosome 2. The different isoforms differ in the carboxyterminal ends, ending with aberrant domain-III and lacking domain IV. Calpain 13 looks similar to the other calpain members in domains I and II, but domain-III lacks the polyglutamic acid stretch that aids in calcium binding, and domain-IV is very different. Sequence alignments place Calpain 13 closest to calpain 14, also found on chromosome 2. |
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| Database links | The links below go to external sites and will open in a new browser window |
| Raised in | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Immunogen affinity purified |
| Storage buffer | Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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| Form | Liquid |
| Concentration | 1.000 mg/ml |
| Storage instructions | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles. |
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| Search PubMed (MEDLINE) for references to Calpain 13 |
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